User:Karmella Haynes/Notebook/BioBrick cloning/2013/01/05: Difference between revisions
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'''PCR''' | '''PCR''' | ||
# V0120, Gib_BBS F (Gib0001) / Gib_BBP R (Gib0002) | # V0120, Gib_BBS F (Gib0001) / Gib_BBP R (Gib0002) | ||
# pSB1A3, Gib_BBS F / Gib_BBP R | # pSB1A3, Gib_BBS F (Gib0001) / Gib_BBP R (Gib0002) | ||
# hPCD, BBP-hPCD fwd (BL9) / hPCD-BL01 rev (BL7) | # hPCD, BBP-hPCD fwd (BL9) / hPCD-BL01 rev (BL7) | ||
# BL01, hPCD-BL01 fwd (BL8) / BL01-BBS rev (BL10) | # BL01, hPCD-BL01 fwd (BL8) / BL01-BBS rev (BL10) | ||
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'''PCR''' | '''PCR''' | ||
# V0120, gg_BBS F / gg_BBP R | # V0120, gg_BBS F (gg0001) / gg_BBP R (gg0002) | ||
# pSB1A3, gg_BBS F / gg_BBP R | # pSB1A3, gg_BBS F (gg0001) / gg_BBP R (gg0002) | ||
# hPCD, gg_BBP-hPCD F | # hPCD, gg_BBP-hPCD F (gg0003) / gg_hPCD-BL01 R (gg0004) | ||
# BL01, gg_BL01 F / gg_BL01-BBS R | # BL01, gg_BL01 F (gg0004) / gg_BL01-BBS R (gg0005) | ||
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | {| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table --> | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. V0120 = | | rowspan="7" | <u>Expected:</u><br>1. V0120 = 3200<br>2. pSB1A3 = 2000<br>3. hPCD = 186<br>4. BL01 = 2520 | ||
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | | rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>10 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | ||
|- | |- |
Revision as of 12:54, 5 January 2013
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01/05/13
Gibson Assembly
Golden Gate Assembly
PCR
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