User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==12/13/12== | ||
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* | * Mnipreps & Digests: KAH111/C-His clones | ||
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| bgcolor=#cfcfcf | Reagent | | bgcolor=#cfcfcf | Reagent | ||
| bgcolor=#cfcfcf | Volume | | bgcolor=#cfcfcf | Volume | ||
| rowspan="7" | <u>Expected:</u><br>1. | | bgcolor=#cfcfcf | Mix (x9) | ||
| rowspan="7" | <! | | rowspan="7" | <u>Expected:</u><br>1-8. KAH111/C-His = 3600, 1123<br>Empty C-His = 3600<br> | ||
| rowspan="7" | <font color=red>Blank gel! (only ladder)</font><br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder] | |||
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| DNA(plasmid) || 2.0 | | DNA(plasmid) || 2.0 || --- | ||
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| 10X buffer || 1.5 | | 10X buffer || 1.5 || 13.5 | ||
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| EcoRI || 1.0 | | EcoRI || 1.0 || 9.0 | ||
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| PstI || 1.0 | | PstI || 1.0 || 9.0 | ||
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| dH<sub>2</sub>O || 9.5 | | dH<sub>2</sub>O || 9.5 || 85.5 | ||
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| || 15 μL | | || 15 μL || | ||
|} | |} | ||
--> 37°C/ ~15 min. | |||
Latest revision as of 22:19, 26 September 2017
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12/13/12
Minipreps
--> 37°C/ ~15 min.
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