The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Karmella's BioBrick Cloning
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>
|
12/03/12
- Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad)
Assemblies
- KAH111/C-His: KAH111/(E/P)/1123 + C-His/(E/P)/3600
- Measure conc.'s
- Behzad: KAH111 = 15 ng/μL
- Behzad: C-His = 57 ng/μL
Ligation |
Plate results (lig : neg crtl) mm/dd/yy
|
1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng |
new BioBrick #:1 (Pick #)
|
2. C-His(E/P)/ 25 ng |
|
|
1 |
2
|
Insert DNA |
1.0 |
---
|
Vector DNA |
0.5 |
0.5
|
2x lgn buf (Roche) |
5.0 |
5.0
|
T4 ligase (NEB) |
1.0 |
1.0
|
dH2O |
2.5 |
3.5
|
|
10 μL |
10 μL
|
|