User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/03: Difference between revisions
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== | ==12/03/12== | ||
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* Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | * Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | ||
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{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/04/12</font> | ||
|- | |- | ||
| 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng || <font color=" | | 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng || <font color="red">KAH111/C-His 0 colonies</font> | ||
|- | |- | ||
| 2. C-His(E/P)/ 25 ng || | | 2. C-His(E/P)/ 25 ng || 0 colonies | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 | | || 1 || 2 | ||
|- | |- | ||
| Insert DNA || 1.0 || --- | | Insert DNA || 1.0 || --- | ||
|- | |- | ||
| Vector DNA || 0.5 || 0.5 | | Vector DNA || 0.5 || 0.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || 5.0 || 5.0 | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| T4 ligase (NEB) || 1.0 || 1.0 | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || 2.5 || 3.5 | | dH<sub>2</sub>O || 2.5 || 3.5 | ||
|- | |- | ||
| || 10 μL || 10 μL | | || 10 μL || 10 μL | ||
|} | |} | ||
Revision as of 16:13, 11 December 2012
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12/03/12
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