User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;">Karmella's BioBrick Cloning</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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== | ==12/03/12== | ||
<!-- Precede finished items with a checkmark ✓ --> | <!-- Precede finished items with a checkmark ✓ --> | ||
* | * Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) | ||
---- | ---- | ||
'''Assemblies''' | '''Assemblies''' | ||
# | # KAH111/C-His: KAH111/(E/P)/1123 + C-His/(E/P)/3600 | ||
* Digests (Fermentas FD) | * Digests (Fermentas FD) | ||
** | ** Already done by Behzad | ||
* Measure conc.'s | * Measure conc.'s | ||
** Behzad: KAH111 = 15 ng/μL | |||
** Behzad: C-His = 57 ng/μL | |||
* | |||
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{| {{table}} cellspacing="3" <!-- Ligations table --> | {| {{table}} cellspacing="3" <!-- Ligations table --> | ||
|- bgcolor=#cfcfcf | |- bgcolor=#cfcfcf | ||
| Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> | | Ligation || <font color="blue"><u>Plate results (lig : neg crtl)</u> 12/04/12</font> | ||
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| 1. | | 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng || <font color="red">KAH111/C-His 0 colonies</font> | ||
|- | |- | ||
| 2. | | 2. C-His(E/P)/ 25 ng || 0 colonies | ||
|} | |} | ||
{| {{table}} cellspacing="3" <!-- Ligation rxn table --> | {| {{table}} cellspacing="3" <!-- Ligation rxn table --> | ||
| || 1 || 2 | | || 1 || 2 | ||
|- | |- | ||
| Insert DNA || | | Insert DNA || 1.0 || --- | ||
|- | |- | ||
| Vector DNA || | | Vector DNA || 0.5 || 0.5 | ||
|- | |- | ||
| 2x lgn buf (Roche) || | | 2x lgn buf (Roche) || 5.0 || 5.0 | ||
|- | |- | ||
| | | T4 ligase (NEB) || 1.0 || 1.0 | ||
|- | |- | ||
| dH<sub>2</sub>O || | | dH<sub>2</sub>O || 2.5 || 3.5 | ||
|- | |- | ||
| || | | || 10 μL || 10 μL | ||
|} | |} | ||
Latest revision as of 22:18, 26 September 2017
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12/03/12
Assemblies
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