User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01

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| dH<sub>2</sub>O || 9.5
| dH<sub>2</sub>O || 9.5
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|-
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| &nbsp; || 15 μL --> 37°C/ ~15 min.
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| &nbsp; || 15.0 μL <br>37°C/ ~15 min.
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Current revision

Karmella's BioBrick Cloning Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)


Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
 Hover name
15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15.0 μL
37°C/ ~15 min.



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