User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01

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(12/01/12)
Current revision (15:58, 2 December 2012) (view source)
(12/01/12)
 
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Line 18: Line 18:
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
{| {{table}} border="1" cellspacing="3" <!-- Digest check rxn. table -->
|-valign="top"
|-valign="top"
-
| bgcolor="grey" | <u>Reagent</u> || <u>Volume</u>
+
| bgcolor=#cfcfcf | Reagent
-
| rowspan="7" | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
+
| bgcolor=#cfcfcf | Volume
-
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="7" width=150 | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
 +
| rowspan="7" | [[Image:KAH120112_gel1.png|250px|Hover name]]<br>15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
|-
|-
| DNA(plasmid) || 2.0 μL
| DNA(plasmid) || 2.0 μL
Line 32: Line 33:
| dH<sub>2</sub>O || 9.5
| dH<sub>2</sub>O || 9.5
|-
|-
-
| &nbsp; || 15 μL --> 37°C/ ~15 min.
+
| &nbsp; || 15.0 μL <br>37°C/ ~15 min.
|}
|}

Current revision

Karmella's BioBrick Cloning Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)



Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
Hover name
15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15.0 μL
37°C/ ~15 min.



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