User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01

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* Compare to empty vector MV2
* Compare to empty vector MV2
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{| class="wikitable" border="0" cellspacing="3" <!-- Digest check rxn. table -->
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{| {{table}} border="0" cellspacing="3" <!-- Digest check rxn. table -->
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| <u>Reagent</u> || <u>Volume</u>  
| <u>Reagent</u> || <u>Volume</u>  

Revision as of 16:19, 1 December 2012

Karmella's BioBrick Cloning Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)



Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
Image:GelImage.jpg
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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