User:Karmella Haynes/Notebook/BioBrick cloning/2012/11/30: Difference between revisions
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'''Assemblies''' | '''Assemblies''' | ||
# KAH3/MV2: (1) KAH3/(X/P)/751 + (2) MV2/( | # KAH3/MV2: (1) KAH3/(X/P)/751 + (2) MV2/(S/P)/4529 | ||
> Digests (Fermentas FD)<br> | > Digests (Fermentas FD)<br> | ||
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| <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 11/31/12</font> | | <u>Ligation</u> || <font color="blue"><u>Plate results (lig : neg crtl)</u> 11/31/12</font> | ||
|- | |- | ||
| 1. KAH3 (X/P)/751, 8 ng + MV2 (S/P)/4529, 25 ng || <font color="blue">KAH3/MV2 | | 1. KAH3 (X/P)/751, 8 ng + MV2 (S/P)/4529, 25 ng || <font color="blue">KAH3/MV2 >10:1 (Pick 2)</font> | ||
|- | |- | ||
| 2. MV2 (S/P)/ 25 ng || | | 2. MV2 (S/P)/ 25 ng || zero colonies | ||
|} | |} | ||
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| || 10 μL || 10 μL || | | || 10 μL || 10 μL || | ||
|} | |} | ||
* Follow quick transformation protocol | |||
* Add 30 uL DH5α-Turbo to each ligation; plate on 100 μg/mL Amp agar | |||
Revision as of 20:56, 19 March 2013
Karmella's BioBrick Cloning | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11/30/12
Assemblies
> Digests (Fermentas FD)
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