User:Karmella Haynes/Notebook/BioBrick cloning/2012/08/22

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08/22/12

  • Minipreps: measure concentration


Minipreps
> Check with E/P digests

Reagent Volume Expected:
1. BB 1 = size
2. BB2 = size
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.


> Measure conc.'s

Sample OD260 260/280 ng/μL
1. FC 2 0.102 1.923 101.5
2. FC 3 0.095 1.933 95.0
3. FC 4 0.072 1.957 72.3




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