User:Karmella Haynes/Notebook/ASU courses/2015/09/30: Difference between revisions

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(Autocreate 2015/09/30 Entry for User:Karmella_Haynes/Notebook/ASU_courses)
 
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==Date==
==Date==
* To-do list
* Banana demo: transformations


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Content
'''Banana scent demo: transfromations'''
* Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell)
* Amp resistant
* Strain 1: BL21
* Strain 2: DH5α-turbo
 
# BL21 + BBa_J45200
# BL21 neg.
# DH5α-turbo + BBa_J45200
# DH5α-turbo neg.
 
 
* Transform with 0.5 uL DNA in 10 uL dH<sub>2</sub>O, or just water (neg)


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Revision as of 18:01, 30 September 2015

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Date

  • Banana demo: transformations

Banana scent demo: transfromations

  • Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell)
  • Amp resistant
  • Strain 1: BL21
  • Strain 2: DH5α-turbo
  1. BL21 + BBa_J45200
  2. BL21 neg.
  3. DH5α-turbo + BBa_J45200
  4. DH5α-turbo neg.


  • Transform with 0.5 uL DNA in 10 uL dH2O, or just water (neg)