User:Karmella Haynes/Notebook/ASU courses/2015/09/30: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ASU Courses</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ASU Courses</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==09/30/15== | ||
* | * Outreach - Banana demo: transformations | ||
---- | ---- | ||
'''Banana Scent demo''' | |||
* For middle schoolers at Phoenix Country Day School | |||
* 57 fifth grade students | |||
'''Banana scent demo: transformations''' | |||
* Banana: pTet-ATF1 (BBa_J45200) (Note: requires isoamyl alcohol to produce smell) | |||
* Amp resistant | |||
* Strain 1: BL21 | |||
* Strain 2: DH5α-turbo | |||
# BL21 + BBa_J45200 | |||
# BL21 neg. | |||
# DH5α-turbo + BBa_J45200 | |||
# DH5α-turbo neg. | |||
* Transform with 0.5 uL DNA in 10 uL dH<sub>2</sub>O, or just water (neg) | |||
* Use 50 μL chem. competent cells for each | |||
* Plate on 100 μg/mL ampicillin | |||
* Grow o/n at 37°C to get colonies | |||
RESULTS | |||
* ~100 colonies on DNA+plates | |||
* 0 - 5 colonies on NEB and DH5α-turbo neg ctrls (respectively) | |||
* Next: Pick colonies for liquid cultures and phenotype tests | |||
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Latest revision as of 01:12, 27 September 2017
ASU Courses | Main project page Next entry |
09/30/15
Banana Scent demo
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