User:Karmella Haynes/Notebook/ASU courses/2014/01/24: Difference between revisions
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Colony''' | |||
| align="center" style="background:#f0f0f0;"|'''1''' | |||
| align="center" style="background:#f0f0f0;"|'''2''' | |||
| align="center" style="background:#f0f0f0;"|'''3''' | |||
| align="center" style="background:#f0f0f0;"|'''4''' | |||
|- | |||
| Conc.||30||227||243||45 | |||
|- | |||
| 260/280||1.9||1.87||1.9||1.9 | |||
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|} | |||
'''Digest plasmids with restriction enzymes and gel electrophoresis (1% agarose) to confirm the assembled construct size''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Plasmid DNA''' | |||
| align="center" style="background:#f0f0f0;"|'''3.0 μL''' | |||
|- | |||
| EcoRI||1.0 μL | |||
|- | |||
| PstI||1.0 μL | |||
|- | |||
| 10x FastDigest buffer + green loading dye||1.5 μL | |||
|- | |||
| dH2O||8.5 μL | |||
|- | |||
| ||15.0 μL total | |||
|- | |||
| Incubate at 37°C for 10 minutes.|| | |||
|- | |||
|} | |||
[[Image:20140131 141956 (2).jpg|400px]] | |||
__NOTOC__ | __NOTOC__ | ||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] |
Revision as of 13:35, 3 February 2014
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01/24/14
Kozak Plasmid Minipreps
Day 1 - Tasks for Behzad:
Day 2 - Tasks for Behzad
Day 3 - Tasks for Behzad
|
Colony | 1 | 2 | 3 | 4 |
Conc. | 30 | 227 | 243 | 45 |
260/280 | 1.9 | 1.87 | 1.9 | 1.9 |
Digest plasmids with restriction enzymes and gel electrophoresis (1% agarose) to confirm the assembled construct size
Plasmid DNA | 3.0 μL |
EcoRI | 1.0 μL |
PstI | 1.0 μL |
10x FastDigest buffer + green loading dye | 1.5 μL |
dH2O | 8.5 μL |
15.0 μL total | |
Incubate at 37°C for 10 minutes. |