User:Karmella Haynes/Notebook/ASU courses/2014/01/24: Difference between revisions
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== | ==01/24/14== | ||
* | * Kozak plasmid minipreps | ||
---- | ---- | ||
'''Kozak Plasmid Minipreps''' | |||
* For BME 100, spring 2014 | |||
* Also for SynBERC Scholars/ Quanta "qOpenPCR" project | |||
Day 1 - Tasks for Behzad: | |||
* Quick-transform Kozak miniprep into DH5α | |||
* Grow on 100μg/mL amp plates overnight. Include zero plasmid negative control | |||
Day 2 - Tasks for Behzad | |||
* Pick 4 colonies, innoculate 4 cultures, 5 ml each | |||
* Grow overnight | |||
Day 3 - Tasks for Behzad | |||
* Pellet the cells in the ancient table-top swing-bucket centrifuge | |||
* Very thoroughly (by vortexing and flicking and pipetting up and down) resuspend cells in appropriate amount of water | |||
* Proceed with miniprep | |||
* Measure DNA concentrations | |||
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Revision as of 16:20, 24 January 2014
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01/24/14
Kozak Plasmid Minipreps
Day 1 - Tasks for Behzad:
Day 2 - Tasks for Behzad
Day 3 - Tasks for Behzad
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