User:Karmella Haynes/Notebook/ASU courses/2013/10/28: Difference between revisions
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==03/18/13== | |||
* Supply list | * Supply list | ||
* Prep for week 2 ( | * Prep for week 2 (10/30/13): PCR, kits and master mixes | ||
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| SYBR green dye (Dr. Garcia) | | SYBR green dye (Dr. Garcia) | ||
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'''Prep for Week 2 | '''Prep for Week 2''' | ||
* Assign biomarker status to fictional patients on Patient Assignment Table | * Assign biomarker status to fictional patients on Patient Assignment Table | ||
* Two patients per team | * Two patients per team | ||
* 36 total teams (18 per section on Wednesday AM/PM) | |||
* Assisted by Alfonso Rivera and Zazu Maloi | |||
* '''Master Mixes'''<br> | * '''Master Mixes'''<br> | ||
** Promega colorless master mix: 8x 50 μL aliquots/ lab group | ** Promega colorless master mix: 8x 50 μL aliquots/ lab group in PCR strip tubes; make 45 total | ||
** DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group) | ** DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group) | ||
Revision as of 14:42, 28 October 2013
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03/18/13
Supply list
Prep for Week 2
DNA/primer mixes: Make ~1/3 "patients" positive for marker
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