User:Karmella Haynes/Notebook/ASU courses/2013/03/18: Difference between revisions
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* Assign biomarker status to fictional patients on Patient Assignment Table | * Assign biomarker status to fictional patients on Patient Assignment Table | ||
* | * Two patients per team | ||
* '''Master Mixes'''<br> | * '''Master Mixes'''<br> | ||
** Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total | ** Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total | ||
** DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group) | ** DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group) | ||
* Put into each rack: 1 set of DNA/ primer mix (labeled +, -, 1, 1, 1, 2, 2, 2), and 2 sets of 4 Promega mix | |||
* Label each rack with patient ID's (followed by 1 or 2) | |||
DNA/primer mixes: Make ~1/3 "patients" positive for marker | DNA/primer mixes: Make ~1/3 "patients" positive for marker | ||
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| Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s''' | | Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s''' | ||
|- | |- | ||
| | | Tues 9:00 || 10 || 20 || 10 || 24 || '''34''' || 10 || 36 || '''46''' | ||
|} | |} | ||
Revision as of 20:36, 18 March 2013
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03/18/13
Supply list
Prep for Week 2, Tuesday
DNA/primer mixes: Make ~1/3 "patients" positive for marker
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