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10/24/12
Prep for Week 2, Wednesday (Section 1)
- Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
- Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)
- Master Mixes
- DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
- To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)
DNA/primer mixes: Make ~1/3 "patients" positive for marker
| Section |
Groups |
Total Patients |
Pos ctrls |
Pos patients x3 reps |
TOTAL (+)'s |
Neg ctrls |
Neg patients x3 reps |
TOTAL (-)'s
|
| Thurs 9:30 |
17 |
34 |
17 |
36 |
53 |
17 |
66 |
83
|
Per Section!
| Reagent |
(+) |
Mix+2 (x55) |
(-) |
Mix+2 (x85)
|
| Primer 1 (P0001) |
1.0 |
55.0 |
1.0 |
85.0
|
| Primer 2 (P0002) |
1.0 |
55.0 |
1.0 |
85.0
|
| Kozak plasmid (54 ng/μL) |
0.2 |
11 |
--- |
---
|
| dH2O |
47.8 |
2629.0 |
48.0 |
4080
|
| |
50.0 |
2750 |
50.0 |
4250
|
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