User:Karmella Haynes/Notebook/ASU courses/2012/10/24: Difference between revisions
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== | ==10/24/12== | ||
* To-do list | * To-do list | ||
---- | ---- | ||
'''Prep for Week 2, Wednesday (Section 1)''' | |||
* Assign biomarker status to fictional patients on Patient Assignment Table (Google doc) | |||
* Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia) | |||
* '''Master Mixes'''<br> | |||
** DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total | |||
** To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group) | |||
DNA/primer mixes: Make ~1/3 "patients" positive for marker | |||
{| class="wikitable" border=1 | |||
|- | |||
| Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s''' | |||
|- | |||
| Thurs 9:30 || 17 || 34 || 17 || 36 || '''53''' || 17 || 66 || '''83''' | |||
|} | |||
Per Section! | |||
{| | |||
| Reagent || (+) || Mix+2 (x55) || (-) || Mix+2 (x85) | |||
|- | |||
| Primer 1 (P0001) || 1.0 || 55.0 || 1.0 || 85.0 | |||
|- | |||
| Primer 2 (P0002) || 1.0 || 55.0 || 1.0 || 85.0 | |||
|- | |||
| Kozak plasmid (54 ng/μL) || 0.2 || 11 || --- || --- | |||
|- | |||
| dH<sub>2</sub>O || 47.8 || 2629.0 || 48.0 || 4080 | |||
|- | |||
| || 50.0 || 2750 || 50.0 || 4250 | |||
|} | |||
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Revision as of 19:19, 24 October 2012
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10/24/12
Prep for Week 2, Wednesday (Section 1)
DNA/primer mixes: Make ~1/3 "patients" positive for marker
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