User:Karmella Haynes/Notebook/ASU courses/2012/10/24

Current revision

ASU Courses

Check results (spot check)

Group 1 1. ctrl 2. ctrl 3. plasmid 4. plasmid 5. plasmid 6. none 7. none 8. none Group 2 1. ctrl 2. ctrl 3. none 4. none 5. none 6. plasmid 7. plasmid 8. plasmid

Prep for Week 2, Wednesday (Sections 2, 3)

Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)

Master Mixes
- DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
- To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)

DNA/primer mixes: Make ~1/3 "patients" positive for marker

Per Section!

@@ Line 8: / Line 8: @@
 ==10/24/12==
-* To-do list
+* Check results for section 1
+* Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes
+----
+'''Check results''' (spot check)
+* Run 5 μL (1/20 sample) on a 1% gel (etBr)
+* Expected size = ~200 - 300
+* Marker: [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg]
+{| class="wikitable" width=500
+|- valign="top"
+| Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid
+| [[Image:KAH102412_gel1.png|250px]]
+|}
@@ Line 24: / Line 40: @@
 DNA/primer mixes: Make ~1/3 "patients" positive for marker
-{| class="wikitable" border=1
+{| {{table}}
 |-
 | Section || Groups || Total Patients || Pos ctrls || Pos patients x3 reps || '''TOTAL (+)'s''' || Neg ctrls || Neg patients x3 reps || '''TOTAL (-)'s'''
@@ Line 35: / Line 51: @@
 Per Section!
-{|
+{| {{table}}
 | Reagent || (+) || Mix+2 (x55) || (-) || Mix+2 (x85)
 |-