User:Karmella Haynes/Notebook/ASU courses/2012/10/24

(Difference between revisions)

Revision as of 03:49, 25 October 2012

ASU Courses

Check results (spot check)

Group 1 1. ctrl 2. ctrl 3. plasmid 4. plasmid 5. plasmid 6. none 7. none 8. none Group 2 1. ctrl 2. ctrl 3. none 4. none 5. none 6. plasmid 7. plasmid 8. plasmid

Prep for Week 2, Wednesday (Sections 2, 3)

Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)

Master Mixes
- DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
- To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)

DNA/primer mixes: Make ~1/3 "patients" positive for marker

Per Section!

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 '''Check results''' (spot check)
-{| class="wikitable"
+{| class="wikitable" width=500
 |- valign="top"
 | Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid