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| | + | | Group 1<br>1. ctrl<br> 2. ctrl<br>3. plasmid<br>4. plasmid<br>5. plasmid<br>6. none<br>7. none<br>8. none<br><br>Group 2<br>1. ctrl<br> 2. ctrl<br>3. none<br>4. none<br>5. none<br>6. plasmid<br>7. plasmid<br>8. plasmid |
| | + | | [[Image:KAH102412_gel1.tiff|250px]] |
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Revision as of 03:32, 25 October 2012
 ASU Courses
|  Main project page
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10/24/12
- Check results ofr section 1
- Prep for week 2 (10/24/12, section 1): real DNA PCR, kits and master mixes
Check results (spot check)
Group 1
1. ctrl
2. ctrl
3. plasmid
4. plasmid
5. plasmid
6. none
7. none
8. none
Group 2
1. ctrl
2. ctrl
3. none
4. none
5. none
6. plasmid
7. plasmid
8. plasmid
| Image:KAH102412 gel1.tiff
|
Prep for Week 2, Wednesday (Sections 2, 3)
- Assign biomarker status to fictional patients on Patient Assignment Table (Google doc)
- Put into baggies: 1 marker, 1 PCR tube rack, 1 trimmed plastic bulb pipette (to be fitted into disposable sterile pipette tips, provided by Garcia)
- Master Mixes
- DONE: Promega colorless master mix: 8x 50 μL aliquots/ lab group (Joseph Mejewski) in PCR strip tubes; 48 total
- To do: DNA/ primer mixes: 1x negative, 1x positive, 3x patient 1, 3x patient 2 (8 samples total/ lab group)
DNA/primer mixes: Make ~1/3 "patients" positive for marker
| Section | Groups | Total Patients | Pos ctrls | Pos patients x3 reps | TOTAL (+)'s | Neg ctrls | Neg patients x3 reps | TOTAL (-)'s
|
| Thurs 9:30 am | 17 | 34 | 17 | 36 | 53 | 17 | 66 | 83
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| Thurs 1:30 pm | 17 | 34 | 17 | 36 | 53 | 17 | 66 | 83
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Per Section!
| Reagent | (+) | Mix+2 (x55) | (-) | Mix+2 (x85)
|
| Primer 1 (P0001) | 1.0 | 55.0 | 1.0 | 85.0
|
| Primer 2 (P0002) | 1.0 | 55.0 | 1.0 | 85.0
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| Kozak plasmid (54 ng/μL) | 0.2 | 11 | --- | ---
|
| dH2O | 47.8 | 2629.0 | 48.0 | 4080
|
| | 50.0 | 2750 | 50.0 | 4250
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