User:Karmella Haynes/BME494 ProjectTemplate: Difference between revisions

Revision as of 11:44, 14 April 2014

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Contents 1 Background & Proposed Application 2 Overview & Purpose 3 Design of a New Device 4 Building the New Device 5 Testing the New Device 6 Human Practices 7 About the Designer 8 References Background & Proposed Application Text legend for the figure Overview & Purpose Text describing the image Design of a New Device Building the New Device Synthetic DNA Layout Resources Testing the New Device A LAC SWITCH MODEL We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated. AN INTERACTIVE MODEL We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system. COLLECTING EMPIRICAL VALUES TO IMPROVE THE MODEL We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions. Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model. Human Practices Danger of Chemicals in Farmlands About the Designer Your Name My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. References [1] Full reference. [2] Full reference. [3] Full reference.

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+==Background & Proposed Application==
-<!-- Below is the code for making a thumbnail image to embed in your text. See BME494_Wiki_Help for help with uploading images to OpenWetWare and using them on your page.-->
+<!-- Below is a template code for making a thumbnail image to embed in your text. See BME494_Wiki_Help for help with uploading images to OpenWetWare and using them on your page.-->
-[[Image:CirEmblemHandwriteLogo2.png|thumb|140px||left|Text describing the image]]
+[[Image:CirEmblemHandwriteLogo2.png|thumb|140px||left|Text legend for the figure]]
+<!-- summarize the classical synthetic device upon which your design is based. If you post an image of a figure from a paper, include an in-text citation in the text legend
 <!-- This is where you can get creative. Think of a practical application for a genetic input/ output switch that is based on the Lac switch you designed. Perhaps it can generate some useful compound instead of RFP/ GFP? Perhaps you could replace the Lac Repressor/ promoter part with something that binds to a different compound to make a sensor? Type a "10,000 foot" overview of your project below this comment line. What is your project's impact on the world? -->