User:Karlena L. Brown/Notebook/PVOH Research/2013/04/05

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/04/05 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
(PVOH Film Preparations)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 +
==OBJECTIVES==
 +
* Begin fluorescence detection through pressure testing analysis of microsphere samples (new modifications & better results)
 +
* Continue to run DSC sample analysis on microsphere samples previously prepared on '''3/29/13'''
 +
* Continue to remove excess oil from microsphere samples prepared on '''03/29/13'''
 +
* Wash microspheres prepared on '''03/29/13''' with hexanes
 +
* Dry microsphere samples rinsed with hexanes in preparation for DSC and X-ray
 +
* Make films containing both clay and PVOH variations to run X-ray analysis
 +
 +
==Straight Pipette: Microsphere Pressure Samples Tested==
 +
'''RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13'''
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Sample Order'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH Type'''
 +
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of Hydrogel Used (g)'''
 +
|-
 +
| 1||50:50||146K||110% CEC Laponite w/ DMHXLBR||0.1883
 +
|-
 +
| 2||90:10||146K||NaMT||0.1742
 +
|-
 +
| 3||90:10||130K||110% CEC Laponite w/ DMHXLBR||0.1404
 +
|-
 +
| 4||90:10||146K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1721
 +
|-
 +
| 5||50:50||146K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1884
 +
|-
 +
| 6||50:50||146K||NaMT||0.1349
 +
|}
 +
==PVOH Film Preparations==
==PVOH Film Preparations==
-
'''Prepared PVOH films in water:'''
+
'''RECALL FILM MAKING PROTOCOL ON 9/02/12'''
-
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
+
 
-
'''(Actual Mass = 0.4934g)'''
+
{| {{table}}
-
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
+
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
-
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
+
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
-
'''(Actual Mass = 0.9965g)'''
+
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
-
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
+
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 +
|-
 +
| NONE||NONE||0.49990||NONE||8.0
 +
|-
 +
| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.25280||0.2468||8.0
 +
|-
 +
| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.45770||0.05030||8.0
 +
|-
 +
| 50:50||110% CEC NaMT w/ DMHXLBR||0.25020||0.25010||8.0
 +
|-
 +
| 90:10||110% CEC NaMT w/ DMHXLBR||0.45020||0.05240||8.0
 +
|}
-
'''Standard PVOH Film Protocol:'''
+
==Notes==
-
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
+
-
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
+
-
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
+
-
'''Notes:'''
 
-
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
 
-
* Heat was reduced when solutions began to boil too rapidly
 
-
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
 

Revision as of 00:34, 15 April 2013

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Begin fluorescence detection through pressure testing analysis of microsphere samples (new modifications & better results)
  • Continue to run DSC sample analysis on microsphere samples previously prepared on 3/29/13
  • Continue to remove excess oil from microsphere samples prepared on 03/29/13
  • Wash microspheres prepared on 03/29/13 with hexanes
  • Dry microsphere samples rinsed with hexanes in preparation for DSC and X-ray
  • Make films containing both clay and PVOH variations to run X-ray analysis

Straight Pipette: Microsphere Pressure Samples Tested

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
150:50146K110% CEC Laponite w/ DMHXLBR0.1883
290:10146KNaMT0.1742
390:10130K110% CEC Laponite w/ DMHXLBR0.1404
490:10146K50% CEC NaMT w/ Bu3HdP+0.1721
550:50146K50% CEC NaMT w/ Bu3HdP+0.1884
650:50146KNaMT0.1349

PVOH Film Preparations

RECALL FILM MAKING PROTOCOL ON 9/02/12

PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL)
NONENONE0.49990NONE8.0
50:5050% CEC NaMT w/ Bu3HdP+0.252800.24688.0
90:1050% CEC NaMT w/ Bu3HdP+0.457700.050308.0
50:50110% CEC NaMT w/ DMHXLBR0.250200.250108.0
90:10110% CEC NaMT w/ DMHXLBR0.450200.052408.0

Notes


Personal tools