User:Karlena L. Brown/Notebook/PVOH Research/2013/03/29

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/03/29 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
(PVOH Film Preparations)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==PVOH Film Preparations==
+
==OBJECTIVES==
-
'''Prepared PVOH films in water:'''
+
* Prepare controlled microsphere samples – PVOH 130K and 146K
-
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
+
* Finish decanting microsphere samples prepared '''03/22/13'''
-
'''(Actual Mass = 0.4934g)'''
+
* Dry microsphere samples rinsed with hexanes '''(03/27/13)''' in preparation for DSC and X-ray
-
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
+
* Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing
-
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
+
-
'''(Actual Mass = 0.9965g)'''
+
-
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
+
-
'''Standard PVOH Film Protocol:'''
+
==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4==
-
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
+
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
-
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
+
-
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
+
-
'''Notes:'''
+
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
-
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
+
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
-
* Heat was reduced when solutions began to boil too rapidly
+
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
-
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
+
 
 +
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 +
 
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''PVOH Type'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Safflower Oil Added (mL)'''
 +
|-
 +
| 130K||0.10400||20||35
 +
|-
 +
| 140K||0.1022||20||35
 +
|}
 +
 
 +
==Notes==
 +
* Samples previously rinsed with hexanes '''03/27/13''' and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
 +
* Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
 +
* The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
 +
* For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.

Revision as of 22:06, 8 April 2013

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Prepare controlled microsphere samples – PVOH 130K and 146K
  • Finish decanting microsphere samples prepared 03/22/13
  • Dry microsphere samples rinsed with hexanes (03/27/13) in preparation for DSC and X-ray
  • Consolidate microsphere samples with dye additive and place in new vials in preparation for diffusion testing

PVOH 146K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH Type PVOH Mass (g) H2O Added (mL) Safflower Oil Added (mL)
130K0.104002035
140K0.10222035

Notes

  • Samples previously rinsed with hexanes 03/27/13 and set aside to air dry in the fume hood were not fully dry. Therefore, these samples were unable to be run for DSC and X-ray diffraction.
  • Also these samples were placed in the lypolizer instrument in attempt to thoroughly dry each sample; however, this was a failed attempt.
  • The lypolizer caused the microspheres to melt together and form hydrogels instead of small spherical samples.
  • For the controlled microsphere samples steadily being prepared, while forming emulsions each sample maintained a very smooth milky cream colored appearance in comparison to the other microsphere samples prepared containing clay additions.



Personal tools