User:Karlena L. Brown/Notebook/PVOH Research/2013/03/27: Difference between revisions

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==Notes==
==Notes==
* On 2013/03/29 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used.   
* On '''03/29/13''' a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used.   
* On 2013/03/29 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.  
* On '''03/29/13''' a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.  


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Revision as of 18:18, 8 April 2013

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OBJECTIVES

  • Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13
  • Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil)

Microspheres: Separation & Vacuum Filtration Procedures

RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13

For the Protocol originally discussed on 03/08/13, some modifications that occurred included: 

     * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes
     * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min
     * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry

Notes

  • On 03/29/13 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used.
  • On 03/29/13 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.


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