User:Karlena L. Brown/Notebook/PVOH Research/2013/03/27

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(Autocreate 2013/03/27 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
(PVOH Film Preparations)
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==PVOH Film Preparations==
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==OBJECTIVES==
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'''Prepared PVOH films in water:'''
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* Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13
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* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
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* Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil)
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'''(Actual Mass = 0.4934g)'''
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==Microspheres: Separation & Vacuum Filtration Procedures==
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* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
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'''RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13'''
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* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
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For the Protocol originally discussed on 03/08/13, some modifications that occurred included:
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'''(Actual Mass = 0.9965g)'''
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      * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes
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* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
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      * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min
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      * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry
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'''Standard PVOH Film Protocol:'''
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* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
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* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
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* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
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'''Notes:'''
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* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
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* Heat was reduced when solutions began to boil too rapidly
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* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
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==Notes==
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* On 2013/03/29 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used. 
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* On 2013/03/29 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.
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Revision as of 20:16, 8 April 2013

PVOH Research Main project page
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OBJECTIVES

  • Remove all excess oil from microsphere samples prepared on 03/20/13 and 03/22/13
  • Clean and dry microsphere samples with hexanes prepared 03/20/13 and 03/22/13 (dissolving excess mineral oil)

Microspheres: Separation & Vacuum Filtration Procedures

RECALL SEPARATION AND VACUUM FILTRATION PROTOCOL ON 3/08/13 For the Protocol originally discussed on 03/08/13, some modifications that occurred included:

     * A vacuum filter system is no longer being used, instead the oil is being decanted off before washing with hexanes
     * Samples are being shaken vigorously for ~ 1 min before allowing to sit and settle for ~ 3 min
     * After decanting off the excess hexane added to dissolve the excess oil in each sample, the samples are being set aside to air dry

Notes

  • On 2013/03/29 a set of controlled microsphere samples will be prepared using only PVOH 130K and 146K. These will be prepared on a 0.05 gram scale to reduce the amount of waste and sample being used.
  • On 2013/03/29 a set of controlled hydrogel samples will be prepared as well using only PVOH 130K and 146K. These will be prepared on a 1 gram scale to reduce the amount of waste and sample being used.


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