User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22

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(PVOH 130K Prepared Microsphere Samples & Dye Preparations 2)
(PVOH 130K Prepared Microsphere Samples & Dye Preparations 2)
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| 50:50||Laponite||0.02590||0.02500||40
| 50:50||Laponite||0.02590||0.02500||40
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| 90:10||NaMT||0.04140||0.01110||40||92
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| 90:10||NaMT||0.04140||0.01110||40
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| 90:10||Laponite||0.04470||0.01100||40
| 90:10||Laponite||0.04470||0.01100||40

Revision as of 19:28, 8 April 2013

PVOH Research Main project page
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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL)
50:50NaMT0.025300.0253040
50:50Laponite0.025900.0250040
90:10NaMT0.041400.0111040
90:10Laponite0.044700.0110040
50:50110% CEC Laponite w/ DMHXLBR0.025500.0250040
90:10110% CEC Laponite w/ DMHXLBR0.042300.0115040
50:5050% CEC NaMT w/ Bu3HdP+0.025400.0250040
90:1050% CEC NaMT w/ Bu3HdP+0.040100.0105040

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
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