User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22

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(PVOH Film Preparations)
(OBJECTIVES)
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==OBJECTIVES==
==OBJECTIVES==
-
* Continue to clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13'''
 
* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
 +
 +
==PVOH 130K Prepared Microsphere Samples & Dye Preparations 2==
 +
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
 +
 +
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
 +
 +
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 +
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
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| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
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|-
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| 50:50||NaMT||0.02530||0.02530||40||165||61
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|-
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| 50:50||Laponite||0.02590||0.02500||40||165||61
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|-
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| 90:10||NaMT||0.04140||0.01110||40||92||109
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|-
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| 90:10||Laponite||0.04470||0.01100||40||92||109
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|-
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| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||40||165||61
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|-
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| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||40||92||109
 +
|-
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| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||40||165||61
 +
|-
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| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||40||92||109
 +
|}
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 +
==Notes==
 +
* Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
 +
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
 +
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
 +
 +
* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==

Revision as of 15:37, 24 March 2013

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50NaMT0.025300.025304016561
50:50Laponite0.025900.025004016561
90:10NaMT0.041400.011104092109
90:10Laponite0.044700.011004092109
50:50110% CEC Laponite w/ DMHXLBR0.025500.025004016561
90:10110% CEC Laponite w/ DMHXLBR0.042300.011504092109
50:5050% CEC NaMT w/ Bu3HdP+0.025400.025004016561
90:1050% CEC NaMT w/ Bu3HdP+0.040100.010504092109

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres

PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.050100.051004016561
90:10110% CEC NaMT w/ DMHXLBR0.010700.091004092109


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