User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22

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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50NaMT0.025300.025304016561
50:50Laponite0.025900.025004016561
90:10NaMT0.041400.011104092109
90:10Laponite0.044700.011004092109
50:50110% CEC Laponite w/ DMHXLBR0.025500.025004016561
90:10110% CEC Laponite w/ DMHXLBR0.042300.011504092109
50:5050% CEC NaMT w/ Bu3HdP+0.025400.025004016561
90:1050% CEC NaMT w/ Bu3HdP+0.040100.010504092109

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres



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