User:Karlena L. Brown/Notebook/PVOH Research/2013/03/22

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(Autocreate 2013/03/22 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
Current revision (20:45, 8 April 2013) (view source)
(PVOH 130K Prepared Microsphere Samples & Dye Preparations 2)
 
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==PVOH Film Preparations==
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==OBJECTIVES==
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'''Prepared PVOH films in water:'''
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* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
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* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
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'''(Actual Mass = 0.4934g)'''
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* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
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* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
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'''(Actual Mass = 0.9965g)'''
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* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
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'''Standard PVOH Film Protocol:'''
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==PVOH 130K Prepared Microsphere Samples & Dye Preparations 2==
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* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
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'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
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* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
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* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
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'''Notes:'''
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'''1μM Rhodamine 6G Dye Concentration (90:10)'''
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* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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* Heat was reduced when solutions began to boil too rapidly
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  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
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* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
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'''1μM Rhodamine 6G Dye Concentration (50:50)'''
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
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| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Safflower Oil Added (mL)'''
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|-
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| 50:50||NaMT||0.02530||0.02530||20||35
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|-
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| 50:50||Laponite||0.02590||0.02500||20||35
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|-
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| 90:10||NaMT||0.04140||0.01110||20||35
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|-
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| 90:10||Laponite||0.04470||0.01100||20||35
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|-
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| 50:50||110% CEC Laponite w/ DMHXLBR||0.02550||0.02500||20||35
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|-
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| 90:10||110% CEC Laponite w/ DMHXLBR||0.04230||0.01150||20||35
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|-
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| 50:50||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.02540||0.02500||20||35
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|-
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| 90:10||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.04010||0.01050||20||35
|}
|}
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__NOTOC__
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==Notes==
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* Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
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  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
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  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
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* Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres

Current revision

PVOH Research Main project page
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OBJECTIVES

  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 130K Prepared Microsphere Samples & Dye Preparations 2

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Safflower Oil Added (mL)
50:50NaMT0.025300.025302035
50:50Laponite0.025900.025002035
90:10NaMT0.041400.011102035
90:10Laponite0.044700.011002035
50:50110% CEC Laponite w/ DMHXLBR0.025500.025002035
90:10110% CEC Laponite w/ DMHXLBR0.042300.011502035
50:5050% CEC NaMT w/ Bu3HdP+0.025400.025002035
90:1050% CEC NaMT w/ Bu3HdP+0.040100.010502035

Notes

  • Originally, samples were being prepared on a 0.1 gram scale; however, since then samples were divided into 1/4. New modifications include:
  # 50:50 clay vs. PVOH samples, the max amount being used is 0.025 grams for each substance
  # 90:10 clay vs. PVOH samples, the max amount being used is 0.01 grams clay and 0.04 grams PVOH
  • Based upon new sample modifications using 1/4 the original ratio, microsphere samples have began to form actual smaller microspheres rather than microgels or connected large clumps of spheres
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