User:Karlena L. Brown/Notebook/PVOH Research/2013/03/20: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Continue to clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13'''
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
'''(Actual Mass = 0.4934g)'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
* Heat was reduced when solutions began to boil too rapidly
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)


'''1μM Rhodamine 6G Dye Concentration (50:50)'''
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
|-
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05010||0.05100||40||165||61
|-
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01070||0.09100||40||92||109
|}
==PVOH 130K Prepared Microsphere Samples & Dye Preparations==
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''PVOH 130K Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
|-
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05070||0.05130||40||165||61
|-
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01090||0.09340||40||92||109
|}
==Notes==
* Unfortunately, DSC samples were not taken before the Rhodamine 6G dye was added to each sample; therefore, these samples must be remade.


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Revision as of 13:20, 24 March 2013

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OBJECTIVES

  • Continue to clean, filter, and separate out the oil from the previously prepared microspheres on 2/22/13
  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 146K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT w/ DMHXLBR 0.05010 0.05100 40 165 61
90:10 110% CEC NaMT w/ DMHXLBR 0.01070 0.09100 40 92 109

PVOH 130K Prepared Microsphere Samples & Dye Preparations

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 130K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT w/ DMHXLBR 0.05070 0.05130 40 165 61
90:10 110% CEC NaMT w/ DMHXLBR 0.01090 0.09340 40 92 109

Notes

  • Unfortunately, DSC samples were not taken before the Rhodamine 6G dye was added to each sample; therefore, these samples must be remade.


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