User:Karlena L. Brown/Notebook/PVOH Research/2013/03/20: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
==PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4==
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
'''(Actual Mass = 0.4934g)'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
* Heat was reduced when solutions began to boil too rapidly
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
|-
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05010||0.05100||40||165||61
|-
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01070||0.09100||40||92||109
|}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''PVOH 130K Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
|-
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05070||0.05130||40||165||61
|-
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01090||0.09340||40||92||109
|}


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Revision as of 13:00, 24 March 2013

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OBJECTIVES

PVOH 146K & 130K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50) 

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT w/ DMHXLBR 0.05010 0.05100 40 165 61
90:10 110% CEC NaMT w/ DMHXLBR 0.01070 0.09100 40 92 109
PVOH vs. Clay Ratio Clay Selection PVOH 130K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50 110% CEC NaMT w/ DMHXLBR 0.05070 0.05130 40 165 61
90:10 110% CEC NaMT w/ DMHXLBR 0.01090 0.09340 40 92 109


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