User:Karlena L. Brown/Notebook/PVOH Research/2013/03/20

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(Autocreate 2013/03/20 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
Current revision (15:20, 24 March 2013) (view source)
(Notes)
 
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==PVOH Film Preparations==
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==OBJECTIVES==
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'''Prepared PVOH films in water:'''
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* Continue to clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13'''
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* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
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* Prepare more microsphere samples expanding upon new previous method developed (new modifications)
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'''(Actual Mass = 0.4934g)'''
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* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
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* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
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'''(Actual Mass = 0.9965g)'''
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* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
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'''Standard PVOH Film Protocol:'''
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==PVOH 146K Prepared Microsphere Samples & Dye Preparations 4==
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* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
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'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
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* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
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* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
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'''Notes:'''
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'''1μM Rhodamine 6G Dye Concentration (90:10)'''
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* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
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  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
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* Heat was reduced when solutions began to boil too rapidly
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  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
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* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
+
 +
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
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 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
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| align="center" style="background:#f0f0f0;"|'''PVOH 146K Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
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| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
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| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
 +
|-
 +
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05010||0.05100||40||165||61
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|-
 +
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01070||0.09100||40||92||109
 +
|}
 +
 +
==PVOH 130K Prepared Microsphere Samples & Dye Preparations==
 +
'''RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13'''
 +
 +
'''1μM Rhodamine 6G Dye Concentration (90:10)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (92μM)V<sub>2</sub>    V<sub>2</sub> = 109μL
 +
 +
'''1μM Rhodamine 6G Dye Concentration (50:50)'''
 +
  M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub>
 +
  1μM (RG6)x 10mL = (165μM)V<sub>2</sub>    V<sub>2</sub> = 61μL
 +
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH 130K Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''Actual Clay Mass (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''H<sub>2</sub>O Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Dye Concentration (μM)'''
 +
| align="center" style="background:#f0f0f0;"|'''Dye Amount Added (μL)'''
 +
|-
 +
| 50:50||110% CEC NaMT w/ DMHXLBR||0.05070||0.05130||40||165||61
 +
|-
 +
| 90:10||110% CEC NaMT w/ DMHXLBR||0.01090||0.09340||40||92||109
 +
|}
 +
 +
==Notes==
 +
* Unfortunately, DSC samples were not taken before the Rhodamine 6G dye was added to each sample; therefore, these samples must be remade.
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Current revision

PVOH Research Main project page
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OBJECTIVES

  • Continue to clean, filter, and separate out the oil from the previously prepared microspheres on 2/22/13
  • Prepare more microsphere samples expanding upon new previous method developed (new modifications)

PVOH 146K Prepared Microsphere Samples & Dye Preparations 4

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 146K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.050100.051004016561
90:10110% CEC NaMT w/ DMHXLBR0.010700.091004092109

PVOH 130K Prepared Microsphere Samples & Dye Preparations

RECALL MICROSPHERE PREPARATION PROTOCOL ON 2/20/13

1μM Rhodamine 6G Dye Concentration (90:10)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (92μM)V2    V2 = 109μL

1μM Rhodamine 6G Dye Concentration (50:50)

  M1V1 = M2V2
  1μM (RG6)x 10mL = (165μM)V2    V2 = 61μL
PVOH vs. Clay Ratio Clay Selection PVOH 130K Mass (g) Actual Clay Mass (g) H2O Added (mL) Dye Concentration (μM) Dye Amount Added (μL)
50:50110% CEC NaMT w/ DMHXLBR0.050700.051304016561
90:10110% CEC NaMT w/ DMHXLBR0.010900.093404092109

Notes

  • Unfortunately, DSC samples were not taken before the Rhodamine 6G dye was added to each sample; therefore, these samples must be remade.


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