User:Karlena L. Brown/Notebook/PVOH Research/2013/03/08: Difference between revisions
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==OBJECTIVES== | ==OBJECTIVES== | ||
* Clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13''' | |||
==Microspheres: Separation & Vacuum Filtration Procedures== | ==Microspheres: Separation & Vacuum Filtration Procedures== | ||
'''Setup of a vacuum filtration system:''' | |||
''' | * Clean 250mL Erlenmeyer Filter Flask | ||
* Large Buchner Funnel | |||
* Whatman #42 Filter Paper | |||
* Long Secure Rubber Hose | |||
# Take a microsphere sample out of its container using a pair of tweezers | |||
# Place a piece of filter paper into the Buchner funnel, then filter the microsphere using the vacuum filtration system setup | |||
# Attach all parts of the apparatus to the vacuum filtrator system | |||
# Place a piece of Whatman #42 filter paper in the Buchner Funnel | |||
# Turn on the vacuum filtrator system | |||
# Check the suction rate to ensure that enough pressure is applied | |||
# Place the microsphere sample into the Buchner funnel | |||
# Allow the vacuum filtration to continue until the microsphere is completely dry | |||
# To continuously remove the oil integrated within the microsphere sample, wash the microspheres with hexanes repeatedly | |||
# Once microspheres completely dehydrate, clean with hexanes and place microsphere samples in new small labeled vial | |||
# Allow microspheres to dry for ~ 1 day | |||
# Wash all glassware and equipment first with H<sub>2</sub>O and then with acetone for later use | |||
==Notes== | ==Notes== | ||
* Many microsphere samples did not exactly precipitate out into smaller tiny spheres, they remained connected. | |||
* Also many microspheres samples appeared to be very absorbent and squishy to touch | |||
* Due to the excess amount of oil used to produce each microsphere sample, several portions and layers of oil had to be decanted off prior to rinsing extensively with hexanes -- samples were decanted by pulling off the oil using Pasteur pipettes | |||
* For many microspheres several portions of hexanes were needed to purify, clean, and remove oil from each sample ~ 25mL (5 portions of 5mL) | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 17:49, 8 April 2013
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OBJECTIVES
Microspheres: Separation & Vacuum Filtration ProceduresSetup of a vacuum filtration system: * Clean 250mL Erlenmeyer Filter Flask * Large Buchner Funnel * Whatman #42 Filter Paper * Long Secure Rubber Hose
Notes
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