User:Karlena L. Brown/Notebook/PVOH Research/2013/03/08

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(Microspheres: Separation & Vacuum Filtration Procedures)
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==OBJECTIVES==
==OBJECTIVES==
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==Hydrogel Pressure Testing Protocol (Straight Pippette)==
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# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample
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# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
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# Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing rubber bulb
 +
# Progressively squeeze the bulb in order to expel the 3mL of H<sub>2</sub>O and apply a pressure to the hydrogels – dispensing  Rhodamine 6G dye (dye leaching)
 +
# Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis
 +
==Microspheres: Separation & Vacuum Filtration Procedures==
==Microspheres: Separation & Vacuum Filtration Procedures==

Revision as of 15:23, 24 March 2013

PVOH Research Main project page
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OBJECTIVES

Hydrogel Pressure Testing Protocol (Straight Pippette)

  1. Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample
  2. Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
  3. Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H2O enter into the pipette by squeezing rubber bulb
  4. Progressively squeeze the bulb in order to expel the 3mL of H2O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching)
  5. Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis

Microspheres: Separation & Vacuum Filtration Procedures

Hydrogel Pressure Samples Tested 3

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
150:50130K110% CEC NaMT w/ DMHXLBR0.1006
250:50130K110% CEC NaMT w/ DMHXLBR0.1022
390:10130KLaponite0.1031
450:50146K110% CEC Laponite w/ DMHXLBR0.1066
590:10130K110% CEC Laponite w/ DMHXLBR0.1083
690:10130K110% CEC NaMT w/ DMHXLBR0.1003
750:50130K110% CEC Laponite w/ DMHXLBR0.1036
850:50146KLaponite0.1016
990:10130K110% CEC NaMT w/ DMHXLBR0.1011
1090:10146K110% CEC NaMT w/ DMHXLBR0.1020
1150:50130KNaMT0.1024
1250:50130K50% CEC NaMT w/ Bu3HdP+0.1015
1350:50146K110% CEC NaMT w/ DMHXLBR0.0998
1490:10130K50% CEC NaMT w/ Bu3HdP+0.1026

Notes


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