User:Karlena L. Brown/Notebook/PVOH Research/2013/03/08

From OpenWetWare

< User:Karlena L. Brown | Notebook | PVOH Research | 2013 | 03(Difference between revisions)
Jump to: navigation, search
(Hydrogel Pressure Samples Tested 3)
Current revision (19:49, 8 April 2013) (view source)
(Notes)
 
(7 intermediate revisions not shown.)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==OBJECTIVES==
==OBJECTIVES==
-
==Hydrogel Pressure Testing Protocol (Straight Pippette)==
+
* Clean, filter, and separate out the oil from the previously prepared microspheres on '''2/22/13'''
-
# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample
+
-
# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette
+
-
# Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing rubber bulb
+
-
# Progressively squeeze the bulb in order to expel the 3mL of H<sub>2</sub>O and apply a pressure to the hydrogels – dispensing  Rhodamine 6G dye (dye leaching)
+
-
# Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis
+
==Microspheres: Separation & Vacuum Filtration Procedures==
==Microspheres: Separation & Vacuum Filtration Procedures==
 +
'''Setup of a vacuum filtration system:'''
 +
  * Clean 250mL Erlenmeyer Filter Flask
 +
  * Large Buchner Funnel
 +
  * Whatman #42 Filter Paper
 +
  * Long Secure Rubber Hose
 +
# Take a microsphere sample out of its container using a pair of tweezers
 +
# Place a piece of filter paper into the Buchner funnel, then filter the microsphere using the vacuum filtration system setup
 +
# Attach all parts of the apparatus to the vacuum filtrator system
 +
# Place a piece of Whatman #42 filter paper in the Buchner Funnel
 +
# Turn on the vacuum filtrator system
 +
# Check the suction rate to ensure that enough pressure is applied
 +
# Place the microsphere sample into the Buchner funnel
 +
# Allow the vacuum filtration to continue until the microsphere is completely dry
 +
# To continuously remove the oil integrated within the microsphere sample, wash the microspheres with hexanes repeatedly
 +
# Once microspheres completely dehydrate, clean with hexanes and place microsphere samples in new small labeled vial
 +
# Allow microspheres to dry for ~ 1 day
 +
# Wash all glassware and equipment first with H<sub>2</sub>O and then with acetone for later use
==Notes==
==Notes==
-
 
+
* Many microsphere samples did not exactly precipitate out into smaller tiny spheres, they remained connected.
 +
* Also many microspheres samples appeared to be very absorbent and squishy to touch
 +
* Due to the excess amount of oil used to produce each microsphere sample, several portions and layers of oil had to be decanted off prior to rinsing extensively with hexanes -- samples were decanted by pulling off the oil using Pasteur pipettes
 +
* For many microspheres several portions of hexanes were needed to purify, clean, and remove oil from each sample ~ 25mL (5 portions of 5mL)
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}
__NOTOC__
__NOTOC__

Current revision

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Clean, filter, and separate out the oil from the previously prepared microspheres on 2/22/13

Microspheres: Separation & Vacuum Filtration Procedures

Setup of a vacuum filtration system:

  * Clean 250mL Erlenmeyer Filter Flask
  * Large Buchner Funnel
  * Whatman #42 Filter Paper
  * Long Secure Rubber Hose
  1. Take a microsphere sample out of its container using a pair of tweezers
  2. Place a piece of filter paper into the Buchner funnel, then filter the microsphere using the vacuum filtration system setup
  3. Attach all parts of the apparatus to the vacuum filtrator system
  4. Place a piece of Whatman #42 filter paper in the Buchner Funnel
  5. Turn on the vacuum filtrator system
  6. Check the suction rate to ensure that enough pressure is applied
  7. Place the microsphere sample into the Buchner funnel
  8. Allow the vacuum filtration to continue until the microsphere is completely dry
  9. To continuously remove the oil integrated within the microsphere sample, wash the microspheres with hexanes repeatedly
  10. Once microspheres completely dehydrate, clean with hexanes and place microsphere samples in new small labeled vial
  11. Allow microspheres to dry for ~ 1 day
  12. Wash all glassware and equipment first with H2O and then with acetone for later use

Notes

  • Many microsphere samples did not exactly precipitate out into smaller tiny spheres, they remained connected.
  • Also many microspheres samples appeared to be very absorbent and squishy to touch
  • Due to the excess amount of oil used to produce each microsphere sample, several portions and layers of oil had to be decanted off prior to rinsing extensively with hexanes -- samples were decanted by pulling off the oil using Pasteur pipettes
  • For many microspheres several portions of hexanes were needed to purify, clean, and remove oil from each sample ~ 25mL (5 portions of 5mL)


Personal tools