User:Karlena L. Brown/Notebook/PVOH Research/2013/02/27: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Continue fluorescence detection through pressure testing analysis of hydrogel samples (new modifications & better results)
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)  
'''(Actual Mass = 0.4934g)'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==Bent Pipette: Hydrogel Pressure Samples Tested 2==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
'''RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13'''  
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
{| {{table}}
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
| align="center" style="background:#f0f0f0;"|'''Sample Order'''
* Heat was reduced when solutions began to boil too rapidly
| align="center" style="background:#f0f0f0;"|'''PVOH vs. Clay Ratio'''
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
| align="center" style="background:#f0f0f0;"|'''PVOH Type'''
| align="center" style="background:#f0f0f0;"|'''Clay Selection'''
| align="center" style="background:#f0f0f0;"|'''Amount of Hydrogel Used (g)'''
|-
| 1||90:10||146K||110% CEC NaMT w/ DMHXLBR||0.1084
|-
| 2||90:10||130K||NaMT||0.1044
|-
| 3||50:50||146K||110% CEC NaMT w/ DMHXLBR||0.1092
|-
| 4||90:10||130K||110% CEC Laponite w/ DMHXLBR||0.1050
|-
| 5||50:50||130K||NaMT||0.1050
|-
| 6||90:10||130K||Laponite||0.1015
|-
| 7||50:50||146K||110% CEC Laponite w/ DMHXLBR||0.1080
|-
| 8||50:50||130K||Laponite||0.1036
|-
| 9||90:10||130K||110% CEC NaMT w/ DMHXLBR||0.1011
|-
| 10||50:50||130K||110% CEC Laponite w/ DMHXLBR||0.1036
|-
| 11||90:10||130K||110% CEC NaMT w/ DMHXLBR||0.1011
|-
| 12||90:10||146K||Laponite||0.1026
|-
| 13||50:50||130K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1052
|-
| 14||50:50||146K||Laponite||0.1045
|-
| 15||90:10||130K||50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup>||0.1145
|}


==Notes==
* The pipettes that provided the best fluorescence results for each sample were both the straight plastic pipette and 90° angle bent pipette
* All samples run maintained a composition incorporating Rhodamine 6G dye
* In comparison, the laponite incorporated hydrogels are squishy, clear, extremely adsorbent, and soft to touch
* The sodium montmorrillonite incorporated hydrogels are hard, dingy, flat, and dull colored (brownish appearance)
* For laponite samples, one can actually see the Rhodamine 6G dye added clearly within each hydrogel sample (reddish pink appearance)
* Also the sodium montmorrillonite samples in most cases began to grow a greenish type mold within the hydrogels
* All samples on 2/27/13 were tested using the bent pipette containing the 90° angle (See figure listed below)
Figure 1. Image of bent pipette used for pressure testing
[[Image:Bent pipette 2 27 13.jpg]]
<br>


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Revision as of 16:16, 14 April 2013

PVOH Research <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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OBJECTIVES

  • Continue fluorescence detection through pressure testing analysis of hydrogel samples (new modifications & better results)

Bent Pipette: Hydrogel Pressure Samples Tested 2

RECALL HYDROGEL PRESSURE TESTING PROTOCOL ON 2/20/13 & FLUORESCENCE ANALYSIS PROCEDURES ON 2/13/13

Sample Order PVOH vs. Clay Ratio PVOH Type Clay Selection Amount of Hydrogel Used (g)
1 90:10 146K 110% CEC NaMT w/ DMHXLBR 0.1084
2 90:10 130K NaMT 0.1044
3 50:50 146K 110% CEC NaMT w/ DMHXLBR 0.1092
4 90:10 130K 110% CEC Laponite w/ DMHXLBR 0.1050
5 50:50 130K NaMT 0.1050
6 90:10 130K Laponite 0.1015
7 50:50 146K 110% CEC Laponite w/ DMHXLBR 0.1080
8 50:50 130K Laponite 0.1036
9 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
10 50:50 130K 110% CEC Laponite w/ DMHXLBR 0.1036
11 90:10 130K 110% CEC NaMT w/ DMHXLBR 0.1011
12 90:10 146K Laponite 0.1026
13 50:50 130K 50% CEC NaMT w/ Bu3HdP+ 0.1052
14 50:50 146K Laponite 0.1045
15 90:10 130K 50% CEC NaMT w/ Bu3HdP+ 0.1145

Notes

  • The pipettes that provided the best fluorescence results for each sample were both the straight plastic pipette and 90° angle bent pipette
  • All samples run maintained a composition incorporating Rhodamine 6G dye
  • In comparison, the laponite incorporated hydrogels are squishy, clear, extremely adsorbent, and soft to touch
  • The sodium montmorrillonite incorporated hydrogels are hard, dingy, flat, and dull colored (brownish appearance)
  • For laponite samples, one can actually see the Rhodamine 6G dye added clearly within each hydrogel sample (reddish pink appearance)
  • Also the sodium montmorrillonite samples in most cases began to grow a greenish type mold within the hydrogels
  • All samples on 2/27/13 were tested using the bent pipette containing the 90° angle (See figure listed below)

Figure 1. Image of bent pipette used for pressure testing


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