User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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# After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | # After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | ||
# Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | # Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | ||
==Hydrogel Pressure Testing Protocol== | |||
# Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions. | |||
# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample. | |||
# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | |||
# Once placing the sample in a Pasteur pipette, attach a ribber bulb to the top of the pipette, and allow 3mL of distilled H2O enter into the pipette by squeezing ribber bulb | |||
# Progressively squeeze the bulb in order to expel the 3mL of H2O and apply a pressure to the hydrogels – dispensing Rhodamine 6G dye (dye leaching) | |||
# Collect the expelled samples into a small 25mL beaker in order to fluorescence detection analysis | |||
==Hydrogel Pressure Samples Tested== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''90:10 PVA MW 146,000-186,000:110% Lamponite hydrogel pressure tests''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| || | |||
|- | |||
| '''Pasteur Pipette Shape'''||'''Amount of Hydrogel Used'''(g) | |||
|- | |||
| Pasteur pipette w/ no modifications||0.1575 | |||
|- | |||
| Pipette stem bent at slight angle < less than 90 degrees||0.1134 | |||
|- | |||
| Pipette stem bent at slight angle < 90 degrees beginning stem of pipette||0.1397 | |||
|- | |||
| Pipette w/ three pockets at top||0.1039 | |||
|- | |||
| Pipette bent twice in the stem||0.1158 | |||
|- | |||
| Pipette bent at top ≈ 90 degree angle w/ pipette sides almost touching||0.1108 | |||
|- | |||
| Pipette with a twist in middle||0.0993 | |||
|- | |||
| Pipette bent twice in the middle||0.1416 | |||
|- | |||
| Pipette w/ two pockets in the top||0.1041 | |||
|- | |||
| Pipette w/ top bent & sides almost touching = 90 degree angle||0.1258 | |||
|- | |||
| Plain Pasteur pipette w/ no modifications: Run #2||0.1210 | |||
|} | |||
==Notes== | ==Notes== |
Revision as of 23:57, 24 February 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
Hydrogel Pressure Testing Protocol
Hydrogel Pressure Samples Tested
Notes |