User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Prepare more microspheres samples expanding upon new previous method developed
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Begin fluorescence detection through pressure testing analysis of hydrogel samples
'''(Actual Mass = 0.4934g)'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==Expanded Method of PVOH Clay Microsphere Preparation==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
# In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H<sub>2</sub>O
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
# Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
# Cool solution for ~ 5 minutes, then remove the stir bar, and add PVOH clay sample to a blender.  
# Afterwards, then add 35mL rather than 25mL of mineral oil to the sample in the blender.
# Blend sample solution prepared in blender for ~ 7 minutes on high to form a more homogeneous mixture / emulsion (creating a suspension of microspheres)
# After 7 minutes, quickly pour solution into several mini 20mL vials and then add some Rhodamine 6G dye to the solution based upon the ratio selection ''' (90:10 vs. 50:50)'''
# Next, quickly freeze the PVOH clay sample immersed in safflower oil in liquid nitrogen for  ~ 5 min. The vial should be held in the liquid nitrogen until the sample is completely frozen throughout.
# After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
# Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to  solution to thaw for 24 hours
 
==Notes==


'''Notes:'''
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
* Heat was reduced when solutions began to boil too rapidly
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)




Revision as of 23:27, 24 February 2013

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OBJECTIVES

  • Prepare more microspheres samples expanding upon new previous method developed
  • Begin fluorescence detection through pressure testing analysis of hydrogel samples

Expanded Method of PVOH Clay Microsphere Preparation

  1. In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H2O
  2. Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample
  3. Cool solution for ~ 5 minutes, then remove the stir bar, and add PVOH clay sample to a blender.
  4. Afterwards, then add 35mL rather than 25mL of mineral oil to the sample in the blender.
  5. Blend sample solution prepared in blender for ~ 7 minutes on high to form a more homogeneous mixture / emulsion (creating a suspension of microspheres)
  6. After 7 minutes, quickly pour solution into several mini 20mL vials and then add some Rhodamine 6G dye to the solution based upon the ratio selection (90:10 vs. 50:50)
  7. Next, quickly freeze the PVOH clay sample immersed in safflower oil in liquid nitrogen for ~ 5 min. The vial should be held in the liquid nitrogen until the sample is completely frozen throughout.
  8. After the addition of the dye and liquid nitrogen freezing, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days
  9. Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours

Notes


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