User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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==Hydrogel Pressure Testing Protocol== | ==Hydrogel Pressure Testing Protocol (Bent Pippette)== | ||
# Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions | # Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions | ||
# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample | # Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample | ||
# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | # Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | ||
# Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing ribber bulb | # Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing ribber bulb |
Revision as of 12:20, 24 March 2013
PVOH Research | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
Important Liquid Nitrogen Safety
PVOH 146K Prepared Microsphere Samples & Dye Preparations 21μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Hydrogel Pressure Testing Protocol (Bent Pippette)
Hydrogel Pressure Samples Tested
Notes
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