User:Karlena L. Brown/Notebook/PVOH Research/2013/02/20: Difference between revisions
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==Important Liquid Nitrogen Safety== | ==Important Liquid Nitrogen Safety== | ||
* Liquid nitrogen is toxic colorless odorless liquid that freely disintegrates into a gas'''(MW: 28.00 g/mole)''' | * Liquid nitrogen is toxic colorless odorless liquid that freely disintegrates into a gas '''(MW: 28.00 g/mole)''' | ||
* Liquid nitrogen is a cryogenic liquid that readily and instantaneously freezes items | * Liquid nitrogen is a cryogenic liquid that readily and instantaneously freezes items | ||
* Avoid all liquid nitrogen skin contact by wearing gloves and goggles | * Avoid all liquid nitrogen skin contact by wearing gloves and goggles | ||
* Liquid nitrogen should be kept in a closed cap double barreled metal tin when not in use to inhibit inhalation and asphyxiation | * Liquid nitrogen should be kept in a closed cap double barreled metal tin when not in use to inhibit inhalation and asphyxiation | ||
* When in use, liquid nitrogen | * When in use, pour liquid nitrogen carefully into double barreled small open metal container -- only small amount necessary | ||
* If spilled, allow substance to evaporate readily -- do not use a paper towel to wipe up substance because it will instantaneously freeze | * If spilled, allow substance to evaporate readily -- do not use a paper towel to wipe up substance because it will instantaneously freeze | ||
* If spilled, avoid standing too close to the spill or inhaling fumes | * If spilled, avoid standing too close to the spill or inhaling fumes | ||
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* When immersing samples into liquid nitrogen, use beaker tongs while standing at a safe distance away from the solution | * When immersing samples into liquid nitrogen, use beaker tongs while standing at a safe distance away from the solution | ||
* Limit the contact of liquid nitrogen on gloves or other body parts due to ability to cause severe frostbite | * Limit the contact of liquid nitrogen on gloves or other body parts due to ability to cause severe frostbite | ||
* To expose of liquid nitrogen, pour solution on the floor in a closed space. Allow substance to freely evaporate into a gas | |||
* Wash hands after removing gloves that were in contact with liquid nitrogen | * Wash hands after removing gloves that were in contact with liquid nitrogen | ||
==PVOH 146K Prepared Samples & Dye Preparations== | ==PVOH 146K Prepared Microsphere Samples & Dye Preparations 2== | ||
'''1μM Rhodamine 6G Dye Concentration (90:10)''' | '''1μM Rhodamine 6G Dye Concentration (90:10)''' | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | ||
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| 50:50||110% CEC Laponite w/ DMHXLBR||0.50960||0.50460||25||165||61 | | 50:50||110% CEC Laponite w/ DMHXLBR||0.50960||0.50460||25||165||61 | ||
|- | |- | ||
| | | 50:50||NaMT||.50250||0.49980||25||92||109 | ||
|} | |} | ||
==Hydrogel Pressure Testing Protocol== | ==Hydrogel Pressure Testing Protocol (Bent Pippette)== | ||
# Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions | # Heat a 9 in. Corning disposable, non-sterile Pasteur pipette using a Bunsen burner in order for the pipette to bend in various directions | ||
# Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample | # Select a hydrogel for pressure analysis and measure out ~ 0.1 grams of the sample | ||
# Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | # Next, using a razor blade cut the hydrogel for testing into small cubes in order to fit into the Pasteur pippette | ||
# Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing ribber bulb | # Once placing the sample in a Pasteur pipette, attach a rubber bulb to the top of the pipette, and allow 3mL of distilled H<sub>2</sub>O enter into the pipette by squeezing ribber bulb | ||
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* Originally the type of oil used to prepare microspheres previously was mineral oil; however, safflower oil was used this time as substitute | * Originally the type of oil used to prepare microspheres previously was mineral oil; however, safflower oil was used this time as substitute | ||
* Safflower oil has a higher freezing point than mineral oil | * Safflower oil has a higher freezing point than mineral oil | ||
* | * Once samples placed in 10mL vials after being removed from blender began to settle, each sample was then sonicated before freezing in liquid nitrogen -- keeping microspheres immersed in organic layer | ||
* For the 90:10 PVOH 146K 110% CEC Laponite w/ DMHXLBR, 45mL vs. 35mL of safflower oil was used | * For the 90:10 PVOH 146K 110% CEC Laponite w/ DMHXLBR, 45mL vs. 35mL of safflower oil was used | ||
* Rhodamine 6G dye maintains high affinity for organics; therefore, Rhodamine 6G dye will be added later to each microsphere newly prepared | * Rhodamine 6G dye maintains high affinity for organics; therefore, Rhodamine 6G dye will be added later to each microsphere newly prepared |
Revision as of 12:20, 24 March 2013
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OBJECTIVES
Expanded Method of PVOH Clay Microsphere Preparation
Important Liquid Nitrogen Safety
PVOH 146K Prepared Microsphere Samples & Dye Preparations 21μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 10mL = (92μM)V2 V2 = 109μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 10mL = (165μM)V2 V2 = 61μL
Hydrogel Pressure Testing Protocol (Bent Pippette)
Hydrogel Pressure Samples Tested
Notes
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