User:Karlena L. Brown/Notebook/PVOH Research/2013/02/15: Difference between revisions
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* Prepare new microsphere samples using new method of preparation | * Prepare new microsphere samples using new method of preparation | ||
==New Method PVOH Clay Microsphere | ==New Method of PVOH Clay Microsphere Preparation== | ||
# In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H<sub>2</sub>O | # In 50mL beaker, dissolve ~ 1.0g total of PVOH 146K or PVOH 130K along with clay additive selected in 25mL hot deionized H<sub>2</sub>O | ||
# Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample | # Place a stir bar in the 50mL beaker and then heat solution at 100°C for ~ 12-15 minutes until complete dissolution of PVOH / clay sample | ||
# Cool solution for ~ 5 minutes, then remove the stir bar, and then add 25mL of mineral oil to the sample | # Cool solution for ~ 5 minutes, then remove the stir bar, and add PVOH clay sample to a blender | ||
# | # Afterwards, then add 25mL of mineral oil to the sample in the blender | ||
# Blend sample solution prepared in blender for ~ 7 minutes on high to form a more homogeneous mixture / emulsion (creating a suspension of microspheres) | |||
# After 7 minutes, | # After 7 minutes, quicklya dd some Rhodamine 6G dye to the solution based upon the ratio selection ''' (90:10 vs. 50:50)''' | ||
# After the addition of dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | # After the addition of the dye, allow the solution to go through freeze / thaw crosslinking process for ~ 2-3 days | ||
# Place microsphere solution in a freezer at -20°C for 24 hours and then remove and | # Place microsphere solution in a freezer at -20°C for 24 hours and then remove and allow to solution to thaw for 24 hours | ||
'''Reference:''' http://www.sciencedirect.com/science/article/pii/S0168365998000893 | '''Reference:''' http://www.sciencedirect.com/science/article/pii/S0168365998000893 | ||
==PVOH 146K Prepared Samples & Dye Preparations== | ==PVOH 146K Prepared Microsphere Samples & Dye Preparations== | ||
'''1μM Rhodamine 6G Dye Concentration''' | '''1μM Rhodamine 6G Dye Concentration (90:10)''' | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | ||
1μM (RG6)x 25mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 271.74μL | 1μM (RG6)x 25mL = (92μM)V<sub>2</sub> V<sub>2</sub> = 271.74μL | ||
'''1μM Rhodamine 6G Dye Concentration''' | '''1μM Rhodamine 6G Dye Concentration (50:50)''' | ||
M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | M<sub>1</sub>V<sub>1</sub> = M<sub>2</sub>V<sub>2</sub> | ||
1μM (RG6)x 25mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 152.00μL | 1μM (RG6)x 25mL = (165μM)V<sub>2</sub> V<sub>2</sub> = 152.00μL | ||
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| 90:10||110% CEC Laponite w/ DMHXLBR||.90100||0.09980||25||92||272 | | 90:10||110% CEC Laponite w/ DMHXLBR||.90100||0.09980||25||92||272 | ||
|} | |} | ||
==Fluorescence Analysis: Diffusion Testing== | |||
# In a 10mL beaker containing one of the hydrogel samples with Rhodamine 6G, place ~ 4mL of H<sub>2</sub>O | |||
# Using a timer, every 15 minutes using a plastic pipette remove ~ 1-2mL of the sample | |||
# In glass cuvette marked for fluorescence, dispense the sample collected into the glass cuvette | |||
# Then place the sample filled cuvette within the sample holder to run fluorescence of the sample | |||
# Once a fluorescence reading has been run of the sample, dispose of the sample collected into a waste container | |||
# After each addition collected and disregarded of the sample, add ~ 1-2mL more H<sub>2</sub>O ensuring enough sample for the next reading | |||
# Repeat this process again for each sample over a 2 hour period | |||
==Notes== | ==Notes== | ||
* Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H<sub>2</sub>O added in order for fluorescent analysis include: | * Hydrogels that completed the freeze / thaw cycle which had ~ 4mL of H<sub>2</sub>O added in order for fluorescent analysis include: | ||
# 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR | |||
# 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR | |||
# 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR | |||
# 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR | |||
* For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels | * For fluorescence analysis, samples were taken every 15 minutes for 2hrs to determine a dye leaching / diffusion rate from hydrogels | ||
* Fluorescent samples run and completed for 2hr diffusion rate determinations: | * Fluorescent samples run and completed for 2hr diffusion rate determinations: | ||
# Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup> | |||
# Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu<sub>3</sub>HdP<sup>+</sup> | |||
# Hydrogel 50:50 PVOH 146K NaMT | |||
# Hydrogel 90:10 PVOH 146K NaMT | |||
# Hydrogel control PVOH 146K | |||
# Hydrogel 90:10 PVOH 146K Laponite | |||
* In regards to the spectra, all samples maintained a quick diffusion rate indicating that dye crosslinking during the freeze / thaw cycle is pertinent when making hydrogels because it slows down the diffusion rate | |||
* For the hydrogels in which the dye was added, but not crosslinked into the material, their diffusion rate was the quickest -- a significant amount of dye leaked out of those hydrogels throughout the 2 hour period | |||
* Comparing the 50:50 vs. the 90:10 ratio of PVOH / clay, the hydrogels with 50:50 ratio had more dye leak out than the 90:10 ratio hydrogels indicating a more effective pressure stimuli | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 20:23, 8 April 2013
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OBJECTIVES
New Method of PVOH Clay Microsphere Preparation
Reference: http://www.sciencedirect.com/science/article/pii/S0168365998000893 PVOH 146K Prepared Microsphere Samples & Dye Preparations1μM Rhodamine 6G Dye Concentration (90:10) M1V1 = M2V2 1μM (RG6)x 25mL = (92μM)V2 V2 = 271.74μL 1μM Rhodamine 6G Dye Concentration (50:50) M1V1 = M2V2 1μM (RG6)x 25mL = (165μM)V2 V2 = 152.00μL
Fluorescence Analysis: Diffusion Testing
Notes
# 50:50 PVOH 130K 110% CEC NaMT w/ DMHXLBR # 90:10 PVOH 130K 110% CEC Laponite w/ DMHXLBR # 50:50 PVOH 130K 110% CEC Laponite w/ DMHXLBR # 90:10 PVOH 130K 110% CEC NaMT w/ DMHXLBR
# Hydrogel 50:50 PVOH 146K 50% CEC NaMT w/ Bu3HdP+ # Hydrogel 90:10 PVOH 146K 50% CEC NaMT w/ Bu3HdP+ # Hydrogel 50:50 PVOH 146K NaMT # Hydrogel 90:10 PVOH 146K NaMT # Hydrogel control PVOH 146K # Hydrogel 90:10 PVOH 146K Laponite
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