User:Karlena L. Brown/Notebook/PVOH Research/2012/11/14: Difference between revisions
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==OBJECTIVES== | ==OBJECTIVES== | ||
* Standardize 0.2M NaHCO<sub>3</sub> solution with KHP | |||
* Conduct swelling examinations of each film | |||
==Standardization Preparation Protocol== | ==Standardization Preparation Protocol== | ||
# In a clean burette, pour in the 0.2M NaHCO<sub>3</sub> solution until it reaches the top | |||
# Turn the stopcock at the bottom of the burette to allow the solution to pass through the tip | |||
# In a clean beaker, run the solution from the burrette until 0.2M NaHCO<sub>3</sub> solution volume reaches the 0.00mL calibration mark | |||
# Record the initial starting point and then turn on the stir plate beneath the burette | |||
# Next, place a new dry 10mL beaker containing a small stir bar onto the stir plate | |||
# While stirring, add ~1g of dry KHP crystals to the beaker | |||
# Next, add ~2 drops of bromothymol blue indicator | |||
# Begin titrating by turning the stopcock on the burette allowing the 0.2M NaHCO<sub>3</sub> solution to steadily stream out | |||
# Slow the pace of the 0.2M NaHCO<sub>3</sub> solution to drops when the PVOH solution begins to turn red in color | |||
# Once the solution completely to turns red, stop titrating by turning the stopcock to '''OFF''' position | |||
# Record the new 0.2M NaHCO<sub>3</sub> volume within the burette | |||
'''REPEAT THE ABOVE STEPS AGAIN FOR THE NEXT TWO KHP SAMPLES''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Sample #''' | |||
| align="center" style="background:#f0f0f0;"|'''Original KHP Amount Dried (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Sample Fraction Cut (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''New KHP Amount Dried (g)''' | |||
|- | |||
| 1||6.8079||1/6||1.13465 | |||
|- | |||
| 2||6.8075||1/6||1.13458 | |||
|- | |||
| 3||6.8076||1/6||1.13460 | |||
|} | |||
==Standardization Calculations== | ==Standardization Calculations== | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Standardization Trial #''' | |||
| align="center" style="background:#f0f0f0;"|'''KHP Amounts (g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Bromothymol Blue Indicator (drops)''' | |||
| align="center" style="background:#f0f0f0;"|'''Initial Burette Volume(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Final Burette Volume(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''KHP Amount Used (mL)''' | |||
|- | |||
| 1||1.13465||2||2.3||10.2||7.9 | |||
|- | |||
| 2||1.12458||2||10.2||19.2||9.0 | |||
|- | |||
| 3||1.13460||2||19.2||29.0||9.8 | |||
|- | |||
| 4||1.13456||2||35.2||46.0||10.8 | |||
|} | |||
==Swelling Test Protocal== | ==Swelling Test Protocal== | ||
==Swelling Test Result Analysis== | ==Swelling Test Result Analysis== | ||
==Notes== | ==Notes== | ||
* Due to so much 0.2M NaHCO<sub>3</sub> solution used for titration, in regards to standardization, the amounts of dried KHP needed to be re-evaluated. Only sixths of the original amounts measured out were be used for standardization. | |||
* Instead of taking three measurements for standardization, four measurements were taken because one trial of results was thrown out due to inaccurate measurements -- indication / equilibrium point was underestimated (trial #1). | |||
Revision as of 23:18, 14 December 2012
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OBJECTIVES
Standardization Preparation Protocol
REPEAT THE ABOVE STEPS AGAIN FOR THE NEXT TWO KHP SAMPLES
Standardization Calculations
Swelling Test ProtocalSwelling Test Result AnalysisNotes
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