User:Karlena L. Brown/Notebook/PVOH Research/2012/11/14

From OpenWetWare

< User:Karlena L. Brown | Notebook | PVOH Research | 2012 | 11(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/11/14 Entry for User:Karlena_L._Brown/Notebook/PVOH_Research)
Current revision (01:18, 15 December 2012) (view source)
(Standardization Preparation Protocol)
 
(10 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==PVOH Film Preparations==
+
==OBJECTIVES==
-
'''Prepared PVOH films in water:'''
+
* Standardize 0.2M NaHCO<sub>3</sub> solution with KHP
-
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
+
* Conduct swelling examinations of each film
-
'''(Actual Mass = 0.4934g)'''
+
-
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
+
-
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
+
-
'''(Actual Mass = 0.9965g)'''
+
-
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker
+
-
'''Standard PVOH Film Protocol:'''
+
==Standardization Preparation Protocol==
-
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
+
# In a clean burette, pour in the 0.2M NaHCO<sub>3</sub> solution until it reaches the top
-
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
+
# Turn the stopcock at the bottom of the burette to allow the solution to pass through the tip
-
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.  
+
# In a clean beaker, run the solution from the burrette until 0.2M NaHCO<sub>3</sub> solution volume reaches the 0.00mL calibration mark
 +
# Record the initial starting point and then turn on the stir plate beneath the burette
 +
# Next, place a new dry 10mL beaker containing a small stir bar onto the stir plate
 +
# While stirring, add ~1g of dry KHP crystals to the beaker
 +
# Next, add ~2 drops of bromothymol blue indicator
 +
# Begin titrating by turning the stopcock on the burette allowing the 0.2M NaHCO<sub>3</sub> solution to steadily stream out
 +
# Slow the pace of the 0.2M NaHCO<sub>3</sub> solution to drops when the PVOH solution begins to turn red in color
 +
# Once the solution completely to turns red, stop titrating by turning the stopcock to '''OFF''' position
 +
# Record the new 0.2M NaHCO<sub>3</sub> volume within the burette
-
'''Notes:'''
+
'''REPEAT THE ABOVE STEPS AGAIN FOR THE NEXT TWO KHP SAMPLES'''
-
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
+
{| {{table}}
-
* Heat was reduced when solutions began to boil too rapidly
+
| align="center" style="background:#f0f0f0;"|'''Sample #'''
-
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)
+
| align="center" style="background:#f0f0f0;"|'''Original KHP Amount Dried (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''Sample Fraction Cut (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''New KHP Amount Dried (g)'''
 +
|-
 +
| 1||6.8079||1/6||1.13465
 +
|-
 +
| 2||6.8075||1/6||1.13458
 +
|-
 +
| 3||6.8076||1/6||1.13460
 +
|}
 +
 
 +
==Standardization Calculations==
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Standardization Trial #'''
 +
| align="center" style="background:#f0f0f0;"|'''KHP Amounts (g)'''
 +
| align="center" style="background:#f0f0f0;"|'''Bromothymol Blue Indicator (drops)'''
 +
| align="center" style="background:#f0f0f0;"|'''Initial Burette Volume(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Final Burette Volume(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''KHP Amount Used (mL)'''
 +
|-
 +
| 1||1.13465||2||2.3||10.2||7.9
 +
|-
 +
| 2||1.12458||2||10.2||19.2||9.0
 +
|-
 +
| 3||1.13460||2||19.2||29.0||9.8
 +
|-
 +
| 4||1.13456||2||35.2||46.0||10.8
 +
|}
 +
 
 +
 
 +
 
 +
==Swelling Test Protocal==
 +
==Swelling Test Result Analysis==
 +
==Notes==
 +
* Due to so much 0.2M NaHCO<sub>3</sub> solution used for titration, in regards to standardization, the amounts of dried KHP needed to be re-evaluated. Only sixths of the original amounts measured out were be used for standardization.
 +
* Instead of taking three measurements for standardization, four measurements were taken because one trial of results was thrown out due to inaccurate measurements -- indication / equilibrium point was underestimated (trial #1).

Current revision

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Standardize 0.2M NaHCO3 solution with KHP
  • Conduct swelling examinations of each film

Standardization Preparation Protocol

  1. In a clean burette, pour in the 0.2M NaHCO3 solution until it reaches the top
  2. Turn the stopcock at the bottom of the burette to allow the solution to pass through the tip
  3. In a clean beaker, run the solution from the burrette until 0.2M NaHCO3 solution volume reaches the 0.00mL calibration mark
  4. Record the initial starting point and then turn on the stir plate beneath the burette
  5. Next, place a new dry 10mL beaker containing a small stir bar onto the stir plate
  6. While stirring, add ~1g of dry KHP crystals to the beaker
  7. Next, add ~2 drops of bromothymol blue indicator
  8. Begin titrating by turning the stopcock on the burette allowing the 0.2M NaHCO3 solution to steadily stream out
  9. Slow the pace of the 0.2M NaHCO3 solution to drops when the PVOH solution begins to turn red in color
  10. Once the solution completely to turns red, stop titrating by turning the stopcock to OFF position
  11. Record the new 0.2M NaHCO3 volume within the burette

REPEAT THE ABOVE STEPS AGAIN FOR THE NEXT TWO KHP SAMPLES

Sample # Original KHP Amount Dried (g) Sample Fraction Cut (g) New KHP Amount Dried (g)
16.80791/61.13465
26.80751/61.13458
36.80761/61.13460

Standardization Calculations

Standardization Trial # KHP Amounts (g) Bromothymol Blue Indicator (drops) Initial Burette Volume(mL) Final Burette Volume(mL) KHP Amount Used (mL)
11.1346522.310.27.9
21.12458210.219.29.0
31.13460219.229.09.8
41.13456235.246.010.8


Swelling Test Protocal

Swelling Test Result Analysis

Notes

  • Due to so much 0.2M NaHCO3 solution used for titration, in regards to standardization, the amounts of dried KHP needed to be re-evaluated. Only sixths of the original amounts measured out were be used for standardization.
  • Instead of taking three measurements for standardization, four measurements were taken because one trial of results was thrown out due to inaccurate measurements -- indication / equilibrium point was underestimated (trial #1).



Personal tools