User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07 - Revision history

Karlena L. Brown: /* UV-Vis Limit of Detection For Salts */

2012-11-30T20:42:02Z

UV-Vis Limit of Detection For Salts

←Older revision		Revision as of 20:42, 30 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
	'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED'''		'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED'''
		+
	To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.		To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Karlena L. Brown: /* UV-Vis Limit of Detection For Salts */

2012-11-30T20:41:42Z

UV-Vis Limit of Detection For Salts

←Older revision		Revision as of 20:41, 30 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
-	To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.	+	'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED'''
		+	To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

	'''Solution of 1.89 μM porphyrin'''		'''Solution of 1.89 μM porphyrin'''

Karlena L. Brown: /* UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations */

2012-11-30T18:43:56Z

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations

←Older revision		Revision as of 18:43, 30 November 2012
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	==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==		==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
-		+	[[Image:Al2%28SO4%293.jpg]]
-		+	<br><br>
		+	[[Image:Fe2%28SO4%293.jpg]]
		+	<br><br>
		+	[[Image:FeSO4.jpg]]
		+	<br><br>
		+	[[Image:Na2SO4.jpg]]

	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Karlena L. Brown: /* UV-Vis Limit of Detection For Salts */

2012-11-30T18:29:54Z

UV-Vis Limit of Detection For Salts

←Older revision		Revision as of 18:29, 30 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
-	~~'''~~To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.~~'''~~	+	To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

-	Solution of 1.89 μM porphyrin	+	'''Solution of 1.89 μM porphyrin'''
	(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)		(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
	V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin		V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin

Karlena L. Brown: /* UV-Vis Limit of Detection For Salts */

2012-11-30T18:29:17Z

UV-Vis Limit of Detection For Salts

←Older revision		Revision as of 18:29, 30 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
		+	'''To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.'''
		+
		+	Solution of 1.89 μM porphyrin
		+	(94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
		+	V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Porphyrine'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
		+	\| align="center" style="background:#f0f0f0;"\|'''FeSO<sub>4</sub> concentration'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Na<sub>2</sub>SO<sub>4</sub> concentration'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume of Water Added [mL]'''
		+	\|-
		+	\| 1.89 µM\|\|1 mM\|\|1 mM\|\|1 mM\|\|1 mM\|\|9.9
		+	\|-
		+	\| 1.89 µM\|\|2 mM\|\|2 mM\|\|2 mM\|\|2 mM\|\|9.8
		+	\|-
		+	\| 1.89 µM\|\|5 mM\|\|5 mM\|\|5 mM\|\|5 mM\|\|9.5
		+	\|-
		+	\| 1.89 µM\|\|10 mM\|\|10 mM\|\|10 mM\|\|10 mM\|\|9
		+	\|}

	==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==		==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==

Karlena L. Brown: /* UV-Vis Limit of Detection For Salts */

2012-11-30T18:17:26Z

UV-Vis Limit of Detection For Salts

←Older revision		Revision as of 18:17, 30 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
		+
	==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==		==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==

Karlena L. Brown: /* UV-Vis LOD Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations */

2012-11-24T09:57:52Z

UV-Vis LOD Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations

←Older revision		Revision as of 09:57, 24 November 2012
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	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
-	==UV-Vis ~~LOD~~ Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==	+	==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==

Karlena L. Brown: /* OBJECTIVES */

2012-11-24T09:57:04Z

OBJECTIVES

←Older revision		Revision as of 09:57, 24 November 2012
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	==OBJECTIVES==		==OBJECTIVES==
-	* Make porphyrin solution with varied SO<sub>4</sub><~~sub~~>2-</~~sub~~> salts to uncover UV-Vis limit of detection (LOD)	+	* Make porphyrin solution with varied SO<sub>4</sub><sup>2-</sup> salts to uncover UV-Vis limit of detection (LOD)
-	* Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub><~~sub~~>2-</~~sub~~> salts	+	* Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub><sup>2-</sup> salts

	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==

Karlena L. Brown: /* OBJECTIVES */

2012-11-24T09:55:43Z

OBJECTIVES

←Older revision		Revision as of 09:55, 24 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==OBJECTIVES==		==OBJECTIVES==
		+	* Make porphyrin solution with varied SO<sub>4</sub><sub>2-</sub> salts to uncover UV-Vis limit of detection (LOD)
		+	* Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub><sub>2-</sub> salts
		+
	==UV-Vis Limit of Detection For Salts==		==UV-Vis Limit of Detection For Salts==
	==UV-Vis LOD Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==		==UV-Vis LOD Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==

Karlena L. Brown: /* PVOH Film Preparations */

2012-11-24T03:15:13Z

PVOH Film Preparations

←Older revision		Revision as of 03:15, 24 November 2012
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~PVOH Film Preparations~~==	+	==OBJECTIVES==
-	~~'''Prepared PVOH films in water:'''~~	+	==UV-Vis Limit of Detection For Salts==
-	* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)	+	==UV-Vis LOD Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
-	~~'''(Actual Mass~~ = 0.~~4934g)'''~~	+
-	* Then, using a graduated cylinder add ~ 3 mL H<sub>2</~~sub>O to the beaker~~	+
-	* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)	+
-	~~'''(Actual Mass~~ = ~~0.9965g)'''~~	+
-	* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker	+

-	~~'''Standard PVOH Film Protocol:'''~~
-	* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
-	* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
-	* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.
-
-	~~'''Notes:'''~~
-	* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
-	* Heat was reduced when solutions began to boil too rapidly
-	* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)