User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07

(Difference between revisions)
 Revision as of 14:29, 30 November 2012 (view source) (→UV-Vis Limit of Detection For Salts)← Previous diff Revision as of 14:29, 30 November 2012 (view source) (→UV-Vis Limit of Detection For Salts)Next diff → Line 11: Line 11: ==UV-Vis Limit of Detection For Salts== ==UV-Vis Limit of Detection For Salts== - '''To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.''' + To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared. - Solution of 1.89 μM porphyrin + '''Solution of 1.89 μM porphyrin''' (94.45 μM)(V1)=(1.89 μM)(10mL) (94.45 μM)(V1)=(1.89 μM)(10mL) V1 = 0.2mL of 94.45 μM porphyrin V1 = 0.2mL of 94.45 μM porphyrin

Revision as of 14:29, 30 November 2012

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OBJECTIVES

• Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
• Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

```  (94.45 μM)(V1)=(1.89 μM)(10mL)
V1 = 0.2mL of 94.45 μM porphyrin
```
 Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL] 1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9 1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8 1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5 1.89 µM 10 mM 10 mM 10 mM 10 mM 9