User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(UV-Vis Limit of Detection For Salts)
(UV-Vis Limit of Detection For Salts)
Line 11: Line 11:
==UV-Vis Limit of Detection For Salts==
==UV-Vis Limit of Detection For Salts==
-
'''To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.'''
+
To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.  
-
Solution of 1.89 μM porphyrin
+
'''Solution of 1.89 μM porphyrin'''
   (94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
   (94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
     V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin
     V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin

Revision as of 13:29, 30 November 2012

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM1 mM1 mM1 mM1 mM9.9
1.89 µM2 mM2 mM2 mM2 mM9.8
1.89 µM5 mM5 mM5 mM5 mM9.5
1.89 µM10 mM10 mM10 mM10 mM9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations


Personal tools