User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07: Difference between revisions

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==PVOH Film Preparations==
==OBJECTIVES==
'''Prepared PVOH films in water:'''
* Make porphyrin solution with varied SO<sub>4</sub><sup>2-</sup> salts to uncover UV-Vis limit of detection (LOD)
* In 10mL beaker, weigh out ~ 0.5 grams PVOH (MW 130,000)
* Take UV-Vis of porphyrin solution with diluted varied SO<sub>4</sub><sup>2-</sup> salts
'''(Actual Mass = 0.4934g)'''
* Then, using a graduated cylinder add ~ 3 mL H<sub>2</sub>O to the beaker
* In another 10mL beaker, weigh out ~ 1.0 gram PVOH (MW 130,000)
'''(Actual Mass = 0.9965g)'''
* Then, using a graduated cylinder add ~ 5 mL H<sub>2</sub>O to the beaker


'''Standard PVOH Film Protocol:'''
==UV-Vis Limit of Detection For Salts==
* After adding and combining PVOH (MW 130,000) in small beakers with H<sub>2</sub>O, add stir bars and prepare to stir solution.
'''STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED'''
* On hot plate, stir and heat both beaker solutions at 70-80°C for ~ 12 min or until PVOH dissolves.
* Once PVOH solids thoroughly dissolve in solution, pour each solution in a Teflon dish to sit, cool, and dry in a fume hood for ~ 1 day.


'''Notes:'''
To find the limit of detection for the 10mM salts (Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.
* An additional 2-3 mL H<sub>2</sub>O was added to each solution to aid the dissolving of the PVOH
* Heat was reduced when solutions began to boil too rapidly
* While transferring solutions to Teflon dishes, loss of sample within the small beakers (sample loss = incomplete sample transfer)


'''Solution of 1.89 μM porphyrin'''
  (94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
    V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Porphyrine'''
| align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
| align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
| align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub> concentration'''
| align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub> concentration'''
| align="center" style="background:#f0f0f0;"|'''Volume of Water Added [mL]'''
|-
| 1.89 µM||1 mM||1 mM||1 mM||1 mM||9.9
|-
| 1.89 µM||2 mM||2 mM||2 mM||2 mM||9.8
|-
| 1.89 µM||5 mM||5 mM||5 mM||5 mM||9.5
|-
| 1.89 µM||10 mM||10 mM||10 mM||10 mM||9
|}
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
[[Image:Al2%28SO4%293.jpg]]
<br><br>
[[Image:Fe2%28SO4%293.jpg]]
<br><br>
[[Image:FeSO4.jpg]]
<br><br>
[[Image:Na2SO4.jpg]]


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Revision as of 13:42, 30 November 2012

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OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED

To find the limit of detection for the 10mM salts (Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin 

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM 1 mM 1 mM 1 mM 1 mM 9.9
1.89 µM 2 mM 2 mM 2 mM 2 mM 9.8
1.89 µM 5 mM 5 mM 5 mM 5 mM 9.5
1.89 µM 10 mM 10 mM 10 mM 10 mM 9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations








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