User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07

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(UV-Vis Limit of Detection For Salts)
(UV-Vis Limit of Detection For Salts)
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==UV-Vis Limit of Detection For Salts==
==UV-Vis Limit of Detection For Salts==
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'''To find the limit of detection for the 10mM salts(Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub>, Al<sub>3</sub>(SO<sub>4</sub>)<sub>3</sub>, FeSO<sub>4</sub>, & Na<sub>2</sub>SO<sub>4</sub>) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.'''
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Solution of 1.89 μM porphyrin
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  (94.45 μM)(V<sub>1</sub>)=(1.89 μM)(10mL)
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    V<sub>1</sub> = 0.2mL of 94.45 μM porphyrin
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Porphyrine'''
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| align="center" style="background:#f0f0f0;"|'''Al<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
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| align="center" style="background:#f0f0f0;"|'''Fe<sub>2</sub>(SO<sub>4</sub>)<sub>3</sub> concentration'''
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| align="center" style="background:#f0f0f0;"|'''FeSO<sub>4</sub> concentration'''
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| align="center" style="background:#f0f0f0;"|'''Na<sub>2</sub>SO<sub>4</sub> concentration'''
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| align="center" style="background:#f0f0f0;"|'''Volume of Water Added [mL]'''
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|-
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| 1.89 µM||1 mM||1 mM||1 mM||1 mM||9.9
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|-
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| 1.89 µM||2 mM||2 mM||2 mM||2 mM||9.8
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|-
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| 1.89 µM||5 mM||5 mM||5 mM||5 mM||9.5
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|-
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| 1.89 µM||10 mM||10 mM||10 mM||10 mM||9
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|}
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==

Revision as of 13:29, 30 November 2012

PVOH Research Main project page
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OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM1 mM1 mM1 mM1 mM9.9
1.89 µM2 mM2 mM2 mM2 mM9.8
1.89 µM5 mM5 mM5 mM5 mM9.5
1.89 µM10 mM10 mM10 mM10 mM9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations


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