User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(UV-Vis Limit of Detection For Salts)
(UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations)
Line 35: Line 35:
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
==UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations==
-
 
+
[[Image:Al2%28SO4%293.jpg]]
-
 
+
<br><br>
 +
[[Image:Fe2%28SO4%293.jpg]]
 +
<br><br>
 +
[[Image:FeSO4.jpg]]
 +
<br><br>
 +
[[Image:Na2SO4.jpg]]
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 14:43, 30 November 2012

PVOH Research Main project page
Previous entry      Next entry

OBJECTIVES

  • Make porphyrin solution with varied SO42- salts to uncover UV-Vis limit of detection (LOD)
  • Take UV-Vis of porphyrin solution with diluted varied SO42- salts

UV-Vis Limit of Detection For Salts

To find the limit of detection for the 10mM salts(Fe2(SO4)3, Al3(SO4)3, FeSO4, & Na2SO4) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

  (94.45 μM)(V1)=(1.89 μM)(10mL)
   V1 = 0.2mL of 94.45 μM porphyrin
Porphyrine Al2(SO4)3 concentration Fe2(SO4)3 concentration FeSO4 concentration Na2SO4 concentration Volume of Water Added [mL]
1.89 µM1 mM1 mM1 mM1 mM9.9
1.89 µM2 mM2 mM2 mM2 mM9.8
1.89 µM5 mM5 mM5 mM5 mM9.5
1.89 µM10 mM10 mM10 mM10 mM9

UV-Vis Spectrums: 1.89μM Porphyrin Solution w/ Varied Salts & Salt Concentrations

Image:Al2(SO4)3.jpg

Image:Fe2(SO4)3.jpg

Image:FeSO4.jpg

Image:Na2SO4.jpg


Personal tools