User:Karlena L. Brown/Notebook/PVOH Research/2012/11/07

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PVOH Research

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OBJECTIVES

Make porphyrin solution with varied SO₄^2- salts to uncover UV-Vis limit of detection (LOD)
Take UV-Vis of porphyrin solution with diluted varied SO₄^2- salts

UV-Vis Limit of Detection For Salts

STOCK SOLUTION OF PORPHYRIN STARTED WITH CONCENTRATION OF 94.45μM, DILUTE SOLUTION TO GIVEN CONCENTRATION LISTED

To find the limit of detection for the 10mM salts (Fe₂(SO₄)₃, Al₃(SO₄)₃, FeSO₄, & Na₂SO₄) originally prepared 10/26/12, UV-Vis was run for each salt at various volumes added to a prepared porphyrin solution. A total of 16 solutions were prepared.

Solution of 1.89 μM porphyrin

  (94.45 μM)(V₁)=(1.89 μM)(10mL)
   V₁ = 0.2mL of 94.45 μM porphyrin

Porphyrine	Al₂(SO₄)₃ concentration	Fe₂(SO₄)₃ concentration	FeSO₄ concentration	Na₂SO₄ concentration	Volume of Water Added [mL]
1.89 µM	1 mM	1 mM	1 mM	1 mM	9.9
1.89 µM	2 mM	2 mM	2 mM	2 mM	9.8
1.89 µM	5 mM	5 mM	5 mM	5 mM	9.5
1.89 µM	10 mM	10 mM	10 mM	10 mM	9